M8413
Monoclonal Anti-Monocyte Chemotactic Protein-2 antibody produced in mouse
clone 35509.11, purified immunoglobulin, lyophilized powder
Synonym(s):
Anti-MCP-2
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About This Item
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
35509.11, monoclonal
form
lyophilized powder
species reactivity
human
technique(s)
indirect ELISA: suitable
neutralization: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CCL8(6355)
General description
Monocyte Chemotactic Protein-2 (MCP-2) belongs to a family of C-C chemotactic cytokines. MCP-1, -3, -4, and -5 are the other five monocyte chemo attractants identified. The MCP cytokines are involved in recruitment of leukocytes to the sites of inflammation, and activate NK cells and CD4+ and CD8+ T lymphocytes. Additionally, MCP cytokines can activate basophils to secrete histamines and also attract eosinophils. MCPs play an important role in pathological conditions such as systemic sclerosis, myocardia and cardiac ischemia. Anti-monocyte chemotactic protein 2 antibody specifically reacts with human MCP-2. The antibody shows <20% cross reactivity with recombinant human MCP-1 and recombinant mouse MIP-1α.
Specificity
Neutralizes the biological activity of recombinant human MCP-2. Shows <20% cross-reactivity with recombinant human MCP-1 and recombinant mouse MIP-1α.
Immunogen
recombinant human MCP-2 expressed in E. coli.
Application
Monoclonal Anti-Monocyte Chemotactic Protein-2 antibody may be used in ELISA at a concentration of 2.0 μg/ml. For immunoblotting a working concentration of 1-2 μg/ml may be used. The antibody is suitable for neutralization reactions; ND50 is 1-4 μg/ml.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose
Preparation Note
Purified using protein A.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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I Clark-Lewis et al.
Journal of leukocyte biology, 57(5), 703-711 (1995-05-01)
Structural analysis of chemokines has revealed that the alpha/beta structural-fold is highly conserved among both the CXC and CC chemokine classes. Although dimerization and aggregation is often observed, the chemokines function as monomers. The critical receptor binding regions are in
Jianli Niu et al.
Clinical science (London, England : 1979), 117(3), 95-109 (2009-07-02)
Many of the major diseases, including cardiovascular disease, are widely recognized as inflammatory diseases. MCP-1 (monocyte chemotactic protein-1) plays a critical role in the development of cardiovascular diseases. MCP-1, by its chemotactic activity, causes diapedesis of monocytes from the lumen
J H W Distler et al.
Rheumatology (Oxford, England), 48(2), 98-103 (2008-11-06)
Activation of the immune system and increased synthesis of extracellular matrix proteins by fibroblasts are hallmarks in the pathogenesis of SSc. The molecular mechanisms underlying the infiltration of inflammatory cells into the skin and the subsequent activation of fibroblasts are
M Weber et al.
Journal of immunology (Baltimore, Md. : 1950), 154(8), 4166-4172 (1995-04-15)
It has been shown that CC chemokines activate basophil and eosinophil leukocytes with different selectivities and patterns of activity. The most effective are monocyte chemotactic protein-1 (MCP-1), a potent stimulus of mediator release in basophils without effects on eosinophils, RANTES
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