Myosin is a 500 kD protein that interacts with actin in muscle and in non-muscle cells. It is characterized with two identical heavy chains (200 kD each) and four light chains (15-20 kD each). Myosin molecules consist of two major regions; the tail (rod) and head. These molecules aggregate into filaments through the tail region and interact with actin and ATP through the head region.
Specificity
Shows a pattern of staining along stress fibers of cultured human and animal fibroblasts.
Immunogen
whole myosin (heavy and light chains) from bovine uterus.
Application
Anti-Myosin (Skeletal and Smooth) antibody produced in rabbit has been used in indirect immunofluorescence labeling.
Biochem/physiol Actions
Myosin plays a vital role in neurosensory and cortical function, membrane vesicle trafficking and determinant partitioning. Myosins with actin-stimulated ATPase activity is essential for various cellular movements. Myosins I and V mediates vesicle translocation. Myosin II with a long rod like tail domain, assembles into a bipolar thick filament and facilitate muscle contraction.
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Plant root hair formation is initiated when specialized elongating root epidermis cells (trichoblasts) assemble distinct domains at the plasma membrane/cell wall cell periphery complexes facing the root surface. These localities show accumulation of expansin and progressively transform into tip-growing root
Myosin: The Actin Motor Protein
Molecular Cell Biology, 10(1), 80-86 (2000)
Root hair formation: F-actin-dependent tip growth is initiated by local assembly of profilin-supported F-actin meshworks accumulated within expansin-enriched bulges
Baluska F, et al.
Developmental Biology, 227(2), 618-632 (2000)
The cell-cell junctions of mammalian testes: II. The lamellar smooth muscle monolayer cells of the peritubular wall are laterally connected by vertical adherens junctions?a novel architectonic cell-cell junction system
Domke LM and Franke WW
Cell and Tissue Research, 375(2), 451-482 (2019)
Myosins: matching functions with motors.
Baker JP and Titus MA.
Current Opinion in Cell Biology, 10(1), 80-86 (1998)
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