Skip to Content
Merck
All Photos(5)

Key Documents

View All Documentation

H6269

Sigma-Aldrich

Hexadecyltrimethylammonium bromide

for molecular biology, ≥99%

Synonym(s):

CTAB, Cetrimonium bromide, Cetyltrimethylammonium bromide, Palmityltrimethylammonium bromide

Sign Into View Organizational & Contract Pricing
All Photos(5)

About This Item

Linear Formula:
CH3(CH2)15N(Br)(CH3)3
CAS Number:
57-09-0
Molecular Weight:
364.45
Beilstein:
3598189
EC Number:
200-311-3
MDL number:
MFCD00011772
UNSPSC Code:
12161902
PubChem Substance ID:
24895734
NACRES:
NA.31
Pricing and availability is not currently available.

grade

for molecular biology

Quality Level

200

description

cationic

Assay

≥99%

form

powder or crystals

mol wt

micellar avg mol wt 62,000

aggregation number

170

pH

5.0-7 (25 °C, 36.4 g/L)

CMC

0.92
1 mM (20-25°C)

mp

248-251 °C (lit.)

solubility

H2O: 1/10 g/mL

HLB

10

suitability

suitable for DNA precipitation

foreign activity

DNAse (Detection)
NICKase Detection
RNAse, none detected

SMILES string

[Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C

InChI

1S/C19H42N.BrH/c1-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20(2,3)4;/h5-19H2,1-4H3;1H/q+1;/p-1

InChI key

LZZYPRNAOMGNLH-UHFFFAOYSA-M

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Hexadecyltrimethylammonium bromide has been used:

  • as a component of DNA extraction buffer[1] and RNA extraction buffer[2]
  • as a constituent of dye dissolution buffer[3]

Biochem/physiol Actions

Hexadecyltrimethylammonium bromide also called cetyltrimethylammonium bromide (CTAB), is a cationic surfactant.[4] CTAB method is an efficient, cost and time effective method of DNA extraction.[5][6]

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2 Oral - STOT SE 3

Target Organs

Gastrointestinal tract, Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

471.2 °F - closed cup

Flash Point(C)

244 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCL4160
BCCL4161
BCCK6046
BCCK6047
BCCK6045

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Xanthoferrin Siderophore Estimation from the Cell-free Culture Supernatants of Different Xanthomonas Strains by HPLC
Pandey SS, et al.
Archives of Virology, 7, e2410-e2410 (2017)
Rolling circle amplification to screen yam germplasm for badnavirus infections and to amplify and characterise novel badnavirus genomes
Bomer M, et al.
Bio-protocol, 8(1), 11-17 (2018)
Weijun Xu et al.
Materials science & engineering. C, Materials for biological applications, 103, 109854-109854 (2019-07-28)
Combination of different therapy modalities with multiple tumoricidal mechanisms has emerged as a promising anticancer strategy. Herein, we reported an aldehyde/catechol-functionalized hyaluronic acid (DAHA) and hydroxyethyl chitosan (HECS) decorated gold nanorod (GNR) platform for combined chemo-photothermal therapy of breast cancer.
The importance of the CTAB surfactant on the colloidal seed-mediated synthesis of gold nanorods
Smith DK and Korgel BA
Langmuir, 24(3), 644-649 (2008)
Investigation of the Orientation of C T A
Karaca S, et al.
Journal of Chemistry, 2013 (2012)
View All Related Papers

Articles

Electronics and Self-Assembly with Single Molecules

Single molecule electronics is the endeavour of constructing electronic circuitry with single molecules as the fundamental building block.

Electronics and Self-Assembly with Single Molecules

Single molecule electronics is the endeavour of constructing electronic circuitry with single molecules as the fundamental building block.

Electronics and Self-Assembly with Single Molecules

Single molecule electronics is the endeavour of constructing electronic circuitry with single molecules as the fundamental building block.

Electronics and Self-Assembly with Single Molecules

Single molecule electronics is the endeavour of constructing electronic circuitry with single molecules as the fundamental building block.

Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. 수용액에 대한 용해도가 36.4g/Liter (100 mM)로 되어 있는데, 1% (29 mM) 수용액을 제작하여 Light Scattering으로 Particle를 측정 시 0.5 um 크기 이상의 particle이 5000 개 /mL이상 측정됩니다. CMC(0.9 mM) 보다 농도가 높아 Micelle이 형성되었나 하는 생각도 있지만, Micelle 대비 입자 크기가 커서 무엇인지 궁금합니다.

    1 answer

    1. For Product H6269 (commonly known as CTAB), the micellar properties have been well studied. The formation of the micelles is dependent on the pH of the solution, as well as the presence of any additives. The CMC has been reported variously as either 0.9 mM or 1.0 mM, so at 29 mM a great majority of the CTAB should be in the form of micelles. The large particles being observed may be aggregates of micelles.

      Running a control sample at a lower CTAB concentration (around 1 mM) would be recommended.

      The micellar properties have been studied by both static light scattering and dynamic light scattering; see:
      https://www.sciencedirect.com/science/article/pii/S0927775714003835#bib0030
      The second paragraph of the Introduction section of that paper cites references 12, 4 and 5.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service