GE28-9924-72
HiScreen™ Capto™ pHenyl (high sub)
Cytiva 28-9924-72, pack of 1 column
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About This Item
UNSPSC Code:
23151817
NACRES:
NA.56
Recommended Products
ligand
phenyl
packaging
pack of 1 column
manufacturer/tradename
Cytiva 28-9924-72
parameter
1.2 - 2.7 ml/min flow rate
bed size
7.7 mm × 100 mm
bed volume
4.7 mL
column I.D.
7.7 mm
matrix
highly cross-linked agarose
avg. part. size
75 μm (d50v)
cleaning
2-14
working range
3-13
capacity
27 mg binding capacity(27 mg BSA /ml (at QB10%))
suitability
suitable for bioprocess medium
General description
Capto™ Phenyl (High sub) and Capto™ Butyl are hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification.
Application
HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resins) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production
Capto™ Phenyl (high sub) is an excellent choice for initial and intermediate step purifications requiring a chromatography medium with medium to high hydrophobicity. Performance and selectivity are similar to that of Phenyl Sepharose™ 6 Fast FLow (high sub). Higher flow rates can be used with Capto™ Phenyl (high sub) during equilibration, wash and regeneration.
Capto™ Phenyl (high sub) is an excellent choice for initial and intermediate step purifications requiring a chromatography medium with medium to high hydrophobicity. Performance and selectivity are similar to that of Phenyl Sepharose™ 6 Fast FLow (high sub). Higher flow rates can be used with Capto™ Phenyl (high sub) during equilibration, wash and regeneration.
Features and Benefits
- Prepacked with Capto™ Phenyl (high sub)
- Excellent for method optimization and parameter screening due to the 10 cm bed height
- Easily connected in series to achieve 20 cm bed height
- Small bed volume for fast results and minimal sample/buffer consumption
- Reproducible results, scalable to BioProcess columns packed with the same media using the same flow velocity
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Capto is a trademark of Cytiva
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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