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E6157

Sigma-Aldrich

Anti-eIF2Bβ (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-EIF-2B, Anti-EIF2B-beta, Anti-EIF2BB, Anti-Eukaryotic translation initiation factor 2B, beta, Anti-Eukaryotic translation initiation factor 2B, subunit 2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~39 kDa

species reactivity

rat, mouse, human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using K562 cell lysates
western blot: 1-2 μg/mL using K562 cell lysates

UniProt accession no.

P49770

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EIF2B2(8892)
mouse ... Eif2b2(217715)
rat ... Eif2b2(84005)

General description

Translation initiation factor eIF-2B subunit beta, eIF2B β is a component of the regulatory subcomplex of eIF2B along with the eIF2Bα and -δ subunits. eIF2Bβ gene is mapped to human chromosome 14q24.3.

Specificity

Anti-eIF2Bβ (C-terminal) specifically recognizes human, mouse, and rat eIF2Bβ.

Application

Anti-eIF2Bβ (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 5-10μg using K562 cell lysates and western blotting at a concentration of 1-2μg/mL using K562 cell lysates.
Anti-eIF2Bβ (C-terminal) antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

The eIF2Bα,-β, and -δ subunits downregulate eIF2B activity in response to the phosphorylation of eIF2 on Ser51. Recent studies have linked inherited mutations in any of the five eIF2B subunits to a fatal human disorder known as childhood ataxia with central nervous system hypomyelination (CACH) or vanishing white matter (VWN) disease.
Translation initiation factor eIF-2B subunit β is a part of a five-subunit complex that catalyzes guanine nucleotide exchange on eIF2. EIF2B2 is associated with the cause of autosomal recessive disorders. Autosomal recessive inherited mutations in each of the five eukaryotic initiation factor 2B (eIF2B) subunits are known to cause white matter abnormalities called eIF2B-related disorders.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yukiko Hata et al.
International journal of clinical and experimental pathology, 7(6), 3355-3362 (2014-07-18)
We report a rare autopsy case of early infantile-onset vanishing white matter disease, with a submicroscopic deletion of 14q24.3, which included EIF2B2 and a missense mutation of EIF2B2 (V85E) of the remaining allele. The patient was a 4-year-old boy, who
G D Pavitt
Biochemical Society transactions, 33(Pt 6), 1487-1492 (2005-10-26)
eIF2B (eukaryotic initiation factor 2B) is a multisubunit protein that is required for protein synthesis initiation and its regulation in all eukaryotic cells. Mutations in eIF2B have also recently been found to cause a fatal human disease called CACH (childhood
W Yang et al.
Molecular and cellular biology, 16(11), 6603-6616 (1996-11-01)
Eukaryotic translation initiation factor 2B (eIF2B) is a five-subunit complex that catalyzes guanine nucleotide exchange on eIF2. Phosphorylation of the alpha subunit of eIF2 [creating eIF2(alphaP]) converts eIF2 x GDP from a substrate to an inhibitor of eIF2B. We showed
Andreas Ohlenbusch et al.
Human mutation, 25(4), 411-411 (2005-03-19)
Autosomal recessive inherited mutations in each of the five eukaryotic initiation factor 2B (eIF2B) subunits are known to cause white matter abnormalities with a wide continuum of clinical signs and severity leading to the concept of eIF2B-related disorders. The clinical

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