The formulation is available on page 3 under DOCUMENTATION or at this link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/947/141/d7777for.pdf
D7777
Dulbecco′s Modified Eagle′s Medium - high glucose
With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
DME, DMEM
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Pricing and availability is not currently available.
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Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
glucose: high
sodium pyruvate: yes
NaHCO3: no
HEPES: no
phenol red: yes
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
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Application
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Quantity
Formulated to contain 13.5 grams of powder per liter of medium.
Reconstitution
Supplement with 3.7 g/L sodium bicarbonate
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
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Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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Hello, can you provide me the exact concentration of L-glutamine and sodium pyruvate present in this DMEM medium.
1 answer-
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