KARPAS 1718
08072401, human spleen (lymphoma), Single round to polymorphic cells growing in suspension
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About This Item
UNSPSC Code:
41106514
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Product Name
KARPAS 1718, 08072401
biological source
human spleen (lymphoma)
growth mode
Suspension
karyotype
Not specified
morphology
Single round to polymorphic cells growing in suspension
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human splenic lymphoma
Cell Line Description
KARPAS 1718 is derived from the peripheral blood of a patient diagnosed with transformed splenic lymphoma with villous lymphocytes
Application
Oncology research
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 8,13
D16S539: 11,13
D5S818: 11,12
D7S820: 8
THO1: 6
TPOX: 8,11
vWA: 14
CSF1PO: 11,13
D13S317: 8,13
D16S539: 11,13
D5S818: 11,12
D7S820: 8
THO1: 6
TPOX: 8,11
vWA: 14
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9 x 105 cells/ml; 5% CO2; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 105 cells/ml. Viability may be poor on resuscitation and may decrease further within the first 48 hours, the culture may appear non-viable at this time. Clusters of cells will eventually appear but this could take 1-2 weeks, during this time large media changes are not recommended. It may take 2-3 weeks for vigorous growth to be established. At subculture perform a cell count and maintain cultures between 3-9 x 105 cells/ml, 5% CO2, 37°c. For cryopreservation of KARPAS 1718, freeze at approximately 2 x 107 cells/ml in 90% FBS + 10% DMSO.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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