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Key Documents

B2661

Sigma-Aldrich

Anti-fd Bacteriophage−Biotin antibody produced in rabbit

~3.5 mg/mL, IgG fraction of antiserum

Synonym(s):

Anti-fd Bacteriophage

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
conjugate:
biotin conjugate
application:
ELISA (i)
clone:
polyclonal
technique(s):
indirect ELISA: 1:500-1:1,000 using 1010-1011 phage/ml coated wells
citations:
6
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

200

conjugate

biotin conjugate

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

concentration

~3.5 mg/mL

technique(s)

indirect ELISA: 1:500-1:1,000 using 1010-1011 phage/ml coated wells

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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Specificity

The antibody binds specifically to phage coat proteins of fd phage or M13 phage and thus may act as a capture antibody when coated directly on multiwell plates or as a primary detection antibody for specifically captured fd or M13 phage. The antibody may be useful in rapidly sorting large phage display libraries (antibody, peptide, etc.) with the expressed proteins fused to either the gene III or to the gene VIII protein of the filamentous phage. The antibody may be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Immunogen

fd bacteriophage

Application

Anti-fd Bacteriophage-Biotin antibody produced in rabbit was used to access immunoglobulin content using bead-based immunoassays.

Physical form

Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000100745
0000100745
0000088047
018M4884
077M4896

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Jussara S Michaloski et al.
Science advances, 2(10), e1600611-e1600611 (2016-11-08)
Receptor tyrosine kinases (RTKs) are key molecules in numerous cellular processes, the inhibitors of which play an important role in the clinic. Among them are the vascular endothelial growth factor (VEGF) family members and their receptors (VEGFR), which are essential
Matthew Robert Tomkins et al.
Sensors (Basel, Switzerland), 15(1), 1047-1059 (2015-01-13)
A detection method that combines electric field-assisted virus capture on antibody-decorated surfaces with the "fingerprinting" capabilities of micro-Raman spectroscopy is demonstrated for the case of M13 virus in water. The proof-of-principle surface mapping of model bioparticles (protein coated polystyrene spheres)
The interface of nanoparticles with proliferating mammalian cells.
Einat Panet et al.
Nature nanotechnology, 12(7), 598-600 (2017-07-07)
Human myoblasts and muscle-derived SP cells.
Grace K Pavlath et al.
Methods in molecular medicine, 107, 97-110 (2004-10-20)
Robert Burger et al.
Lab on a chip, 12(7), 1289-1295 (2012-02-16)
We present a novel centrifugal microfluidic platform for the highly efficient manipulation and analysis of particles for applications in bead-based assays. The platform uses an array of geometrical V-cup barriers to trap particles using stopped-flow sedimentation under highly reproducible hydrodynamic
View All Related Papers

Questions

1–8 of 8 Questions  

  1. The description states Product B2661, Anti-fd Bacteriophage, may be used for staining DNA, chromosomes and nuclei, and for fluorescence microscopy or flow cytometry.  Are there other uses for the product?

    1 answer

    1. The product has also been used for the detection of apoptosis, applied as a label to live cells, as a component of BrdU cell cycle investigation.

      Helpful?

  2. Which is more cell permeable, H33342 or H33258?

    1 answer

    1. H33342 has been found to be about 10 times more cell-permeable than H33258. See Science, 282(5387), 296-298, (1998).

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  3. What is the expected solution stability for aqueous solutions of Product B2661, Anti-fd Bacteriophage?

    1 answer

    1. Aqueous solutions are stable for 1 month if kept in the dark at 2-8 °C.

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  4. When using Product B2661, Anti-fd Bacteriophage, which is the preferred Hoechst stain for chromosomes?

    1 answer

    1. Hoechst No. 33258 and not Hoechst No. 33342 is the preferable dye for chromosomal staining.

      Helpful?

  5. Will chromosomes that are dividing or replicating stain with Product B2661, Anti-fd Bacteriophage?

    1 answer

    1. No; chromosomes that are dividing or replicating will not stain with this dye.

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  6. What is the specificity for H33342?

    1 answer

    1. The bisBenzimide Hoechst 33342 is a specific stain for AT-rich regions of double-stranded DNA and has been shown to displace several known DNA intercalators.  Reference: Loontiens, F. G., et al., Binding characteristics of Hoechst 33258 with calf thymus DNA, poly[d(A-T)] and d(CCGGAATTCCGG): multiple stoichiometries and determination of tight binding with a wide spectrum of site affinities. Biochemistry, 29, p. 9029 (1990).

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  7. What is the pH of Product B2661, Anti-fd Bacteriophage, in solution?

    1 answer

    1. There is no pH test specification for product B2261 on a lot-to-lot basis.  A one-time measurement of the pH of a 2% solution was 1.9. It has been observed that this material will precipitate from phosphate buffer solutions.

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  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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