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A9810

Sigma-Aldrich

Amyloid β Protein Fragment 1-42

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About This Item

Empirical Formula (Hill Notation):
C203H311N55O60S
CAS Number:
107761-42-2
Molecular Weight:
4514.04
MDL number:
MFCD00163049
UNSPSC Code:
12352202
NACRES:
NA.32
Pricing and availability is not currently available.

Assay

≥95% (HPLC)

Quality Level

200

form

powder

UniProt accession no.

P05067

storage temp.

−20°C

SMILES string

S(CC[C@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(

InChI

1S/C203H311N55O60S/c1-28-106(20)164(195(310)220-91-149(267)228-130(71-98(4)5)181(296)238-129(66-70-319-27)179(294)251-158(100(8)9)193(308)218-87-146(264)215-88-151(269)250-160(102(12)13)198(313)255-163(105(18)19)199(314)258-165(107(21)29-2)200(315)227-112

Gene Information

human ... APP(351)

Amino Acid Sequence

Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala

General description

Amyloid β Protein is produced from amyloid-β precursor protein (APP). It consists of two C terminal variants, such as a long tailed Aβ 1-42 and a short tailed Aβ 1-40.[1] APP is located on human chromosome 21q21.3.[2]

Application

Amyloid β Protein Fragment 1-42 has been used:
  • for the preparation of Aβ 1-42 oligomer[3][4]
  • for western blot analysis[5]
  • for the interference test of immunomagnetic reduction (IMR) plasma Aβ42 assay[6]
  • to study the effects of resveratrol on Aβ 1-42-induced impairment of spatial learning, memory and synaptic plasticity[7]
  • to investigate the effect of Aβ in epithelial cell culture[1]

Biochem/physiol Actions

Amyloid β Protein Fragment 1-42 (Aβ 1-42) has antioxidant and neuroprotective properties.[8] Accumulation of amyloid β Protein is associated with Alzheimer′s disease (AD) and Down Syndrome.[9] Aβ 1-42 regulates cholesterol transport[10] and may function as a transcription factor.[11] It may possess anti-inflammatory and antimicrobial properties.[12]
The predominant fragment of amyloid β-protein found in the brains of patients with Alzheimer′s disease and Down′s syndrome.

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000388520
0000387190
0000368432
0000336675
0000331858

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Plasma Amyloid-beta(Abeta 42) Correlates with Cerebrospinal Fluid Abeta 42 in Alzheimer?s Disease
Teunissen CE, et al.
Journal of Alzheimer'S Disease, 1-7 (2018)
Magnesium Ions Inhibit the Expression of Tumor Necrosis Factor alpha and the Activity of gamma-secretase in a beta-amyloid Protein-dependent Mechanism in APP/PS1 Transgenic Mice
Yu X, et al.
Frontiers in Molecular Neuroscience, 11 (2018)
The role of Abeta circRNA in Alzheimer? s disease: alternative mechanism of Abeta biogenesis from Abeta circRNA translation
Mo D, et al.
bioRxiv, 260968-260968 (2018)
beta-amyloid expression in age-related cataract lens epithelia and the effect of beta-amyloid on oxidative damage in human lens epithelial cells
Xu J, et al.
Molecular Vision, 23, 1015-1015 (2017)
Kyunghee Byun et al.
PloS one, 7(5), e37917-e37917 (2012-06-05)
Advanced glycation end products (AGEs) have long been considered as potent molecules promoting neuronal cell death and contributing to neurodegenerative disorders such as Alzheimer's disease (AD). In this study, we demonstrate that AGE-albumin, the most abundant AGE product in human
View All Related Papers

Questions

1–10 of 10 Questions  

  1. After dissolving in DMSO, I want to dilute it with saline (finally 1ml/1mg) and use it for i.c.v. injection in mice. How many µl of 0.4-4 mM DMSO per 0.1 mg should I dissolve in?

    1 answer

    1. This product is soluble in DMSO at 0.4 - 4 mM, as stated in the product datasheet. This equates to 1.8 - 18 mg/mL in DMSO. This stock solution may be diluted with aqueous buffers, such as PBS or saline, to the desired working concentration. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdf

      Helpful?

  2. What is the difference between the AB peptide (1-42)(PP69) and AB protein fragment (1-42)(A9810)?

    1 answer

    1. These products are functionally the same, but are different brands. The PP69 is a Calbiochem product, while the A9810 is from Sigma-Aldrich. The testing specifications are slightly different, however both offer a minimum purity of 95%. The A9810 is lyophilized from a solution of water and TFA. The PP69 is lyophilized from a solution of 50 mM Tris-HCl. Please see the links below to review the product datasheet for each:
      PP69 Calbiochem:
      https://www.emdmillipore.com/product/-Amyloid-Peptide-1-42-Human,EMD_BIO-PP69#anchor_PDS
      A9810 Sigma-Aldrich:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdf

      Helpful?

  3. It is instructed to dissolve the product in Ammonium Hydroxide solution, but what should be the molarity / Concentration of the ammonium hydroxide solution

    1 answer

    1. This product can be solubilized in ammonium hydroxide at a pH greater than 9. The concentration is not reported. It is recommended to prepare at a minimum 1 mg/mL concentration.

      Helpful?

  4. How much ammonium hydroxide (pH >9) should be added to the 0,1 mg vial ? Is it possible to vortex the vial ?

    1 answer

    1. It is recommended to prepare a solution at a minimum of 1 mg/mL of ammonium hydroxide. Vertexing the vial is not necessary.

      Helpful?

  5. in which solvent it should be dissolved ?

    1 answer

    1. As per the product information sheet, this product is soluble in ammonium hydroxide, pH >9, or in DMSO at 0.4–4 mM. Solutions can then be diluted into aqueous buffers such as10 mM sodium phosphate for use. Other reported solvents include hexafluoroisopropanol, acetonitrile, and acetic acid.

      See the link below to review the product information sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdf

      Helpful?

  6. Is the mouse protein fragment the same as the human protein fragment in Product A9810, Amyloid β Protein Fragment 1-42?

    1 answer

    1. The homology is 93% by identity, 95% positives...  no gaps

      Helpful?

  7. What is the source of Product A9810, Amyloid β Protein Fragment 1-42?

    1 answer

    1. This protein fragment is a synthetic organic.  The gene for this peptide is located on human chromosome 21.

      Helpful?

  8. What is the secondary structure of Product A9810, Amyloid β Protein Fragment 1-42?

    1 answer

    1. The secondary structure of this fragment will exist as a mixture of B-sheet, a-helix, and random coil structures dependent upon the solution conditions as described in J. Mol. Biol., 225, 1075-1083, 1992

      Helpful?

  9. What is the pI of Product A9810, Amyloid β Protein Fragment 1-42?

    1 answer

    1. The pI as calculated from the amino acid sequence for Product A9810, Amyloid β Protein Fragment 1-42, is 5.31.

      Helpful?

  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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