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A3313

Sigma-Aldrich

Anti-Human Polyvalent Immunoglobulins (α, γ and μ-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

Human immunoglobulins are glycoproteins that regulate immune responses to infections and allergies. Anti-human polyvalent immunoglobulins can be used for serological analyses in diseased population groups. These antibodies may also be used for studying xenotransplantation models and immunological disorders such as rheumatoid arthritis. Anti-Human Polyvalent Immunoglobulins (α, γ and μ-chain specific)-Alkaline Phosphatase antibody is specific for human IgG, IgA and IgM when tested against human IgA, IgG, IgM, Bence Jones κ, and λ myeloma proteins.

Immunogen

Purified human IgG, IgA and IgM

Application

Anti-Human Polyvalent Immunoglobulins (α, γ and μ-chain specific)-Alkaline Phosphatase antibody is suitable for use in western blot and immunostaining techniques.
The presence of IgG was detected in normal human sera using alkaline phosphatase-conjugated goat anti-human polyvalent IgG (chain specific) by ELISA at a concentration of 1:1000. An ELISA assay was performed using alkaline phosphatase-conjugated goat anti-human IgG polyvalent antibody to detect natural antibodies to KLH in human sera.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 10 mM glycine, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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John S Spencer et al.
Clinical and vaccine immunology : CVI, 18(2), 260-267 (2010-12-24)
A simple serodiagnostic test based on the Mycobacterium leprae-specific phenolic glycolipid I(PGL-I), for individuals with leprosy is nearly universally positive in leprosy patients with high bacillary loads but cannot be used as a stand-alone diagnostic test for the entire spectrum
Luisa Fernanda Duarte et al.
Colombia medica (Cali, Colombia), 45(2), 61-66 (2014-08-08)
To compare the diagnostic performance of seven methods to determine Trypanosoma cruzi infection in patients with chronic Chagas disease. Analytical study, using the case-control design, which included 205 people (patients with Chagasic cardiomyopathy, n=100; control group, n=105). Three enzyme linked
Angela Barone et al.
The Journal of biological chemistry, 288(14), 10035-10050 (2013-02-14)
Due to their pluripotency and growth capability, there are great expectations for human embryonic stem cells, both as a resource for functional studies of early human development and as a renewable source of cells for use in regenerative medicine and
S A Pesoa et al.
Autoimmunity, 4(3), 171-179 (1989-01-01)
The distribution of frequencies of HLA-DR alloantigens in HLA-DR4 negative subjects was determined in patients with Rheumatoid arthritis (RA) and normal individuals. An increased incidence of HLA-DR1 alloantigen in DR4 negative RA patients (45.9%) compared with DR4 negative healthy controls
Béatrice Volney et al.
Acta tropica, 82(1), 11-23 (2002-03-21)
This paper describes a sero-epidemiological study of malaria prevalence in French Guiana. An immunofluorescence assay and an enzyme-linked immunosorbent assay were used to detect antibodies against blood-stage antigens and synthetic peptides mimicking the repetitive epitope of the sporozoites of Plasmodium

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