ATG3 is a member of the autophagy-related protein family and is involved in the formation of the autophagosome.
Autophagy-related protein 3 (ATG3) is a member of the autophagy-related protein family. It is a mammalian homolog of yeast Apg3p/ Atg3 is ubiquitously expressed in human tissues.
Immunogen
synthetic peptide corresponding to amino acids 17-32 of human Atg3, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.
Application
Anti-ATG3 antibody produced in rabbit has been used in western blotting and immunoprecipitation.
Biochem/physiol Actions
Autophagy-related protein 3 (ATG3) is involved in the formation of the autophagosome. the mammalian homolog of yeast Apg3p/Aut1p, is an E2-like enzyme that catalyzes the conjugation reaction between Atg8 and phosphatidylethanolamine (PE). Golgi-associated ATPase enhancer of 16 kDa (GATE-16), GABAA receptor-associated protein (GABARAP), and microtubule-associated protein light chain 3 (MAP-LC3) are substrates for Atg3. MAP-LC3 is the preferred substrate. Atg3 interacts with Atg7 to form an E1-E2 complex, and with Atg12, which is a substrate for Atg7 but not for Atg3. Moreover, overexpression of Atg3 facilitates the formation of the Atg12-Atg5 conjugate.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
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Human ATG4 autophagy proteases counteract attachment of ubiquitin-like LC3/GABARAP proteins to other cellular proteins
Agrotis A, et al.
The Journal of Biological Chemistry, 294(34), 12610-12621 (2019)
ATG12-ATG3 interacts with Alix to promote basal autophagic flux and late endosome function
Murrow L, et al.
Nature Cell Biology, 17(3), 300-300 (2015)
The transcription and translation landscapes during human cytomegalovirus infection reveal novel host-pathogen interactions
Tirosh O, et al.
PLoS Pathogens, 11(11), e1005288-e1005288 (2015)
Structural basis of ATG3 recognition by the autophagic ubiquitin-like protein ATG12
Metlagel Z, et al.
Proceedings of the National Academy of Sciences of the USA, 110(47), 18844-18849 (2013)
Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p
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