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957D

Sigma-Aldrich

DAB Substrate Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.32
Pricing and availability is not currently available.

description

For In Vitro Diagnostic Use in Select Regions

Quality Level

500

form

aqueous solution

packaging

pkg of 1000 tests (957D-60)
pkg of 150 tests (957D-50)
pkg of 2000 tests (957D-30)
pkg of 500 tests (957D-20)
pkg of 5000 tests (957D-40)

manufacturer/tradename

Cell Marque®

technique(s)

immunohistochemistry: suitable

storage temp.

2-8°C

General description

DAB Substrate Kit is intended for use in immunohistochemistry (IHC) staining protocols. This reagent is designed to detect target antigens in formalin-fixed, paraffin-embedded tissue sections when used in conjunction with primary antibodies and appropriate secondary antibody conjugates in the IHC staining process.When in the presence of HRP polymer, DAB produces a brown precipitate that can be readily visualized using light microscopy. Kits contain: DAB chromogen and DAB buffer substrate.

Quality

United States - IVD
Canada - IVD
European Union - IVD
Japan - RUO

Physical form

Ready to use reagent

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Other Notes

For Technical Service please contact: 800-665-7284 or email: [email protected]

Legal Information

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H341,H350

Hazard Classifications

Carc. 1B - Muta. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000137739
0000162184
0000293930
0000384003
0000161181

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Questions

1–3 of 3 Questions  

  1. how to prep DAB?

    1 answer

    1. it is one drop of chromogen to 1ml of substrate buffer.

      Helpful?

  2. Is the absence of color normal when 1 drop of chromagen and 1 ml of substrate are used, given the usual light purple color?

    1 answer

    1. Ensure thorough mixing of the chromagen before combining. The color outcome is influenced by the chromogen's age. A lack of color typically suggests very recent or fresh DAB, which has not undergone oxidation.

      Helpful?

  3. What is the volume for "1 drop" of DAB chromogen?

    1 answer

    1. The volume used to prepare 1ml of DAB would be 0.006mls, rounded to 0.01mls based on calculations for the number of tests in the kit 957D-20.

      Helpful?

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