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36438

Sigma-Aldrich

Fluorescein diacetate 6-isothiocyanate

≥90% (HPLC)

Synonym(s):

Diacetyl-6-FITC, Fluorescein-6-isothiocyanate diacetate

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About This Item

Empirical Formula (Hill Notation):
C25H15NO7S
CAS Number:
890090-49-0
Molecular Weight:
473.45
MDL number:
MFCD08276996
UNSPSC Code:
12352200
PubChem Substance ID:
57649528
NACRES:
NA.32

Assay

≥90% (HPLC)

form

powder

fluorescence

λex 496 nm; λem 517 nm in 0.1 M Tris pH 8.0, esterase

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)N=C=S)c1

InChI

1S/C25H15NO7S/c1-13(27)30-16-4-7-19-22(10-16)32-23-11-17(31-14(2)28)5-8-20(23)25(19)21-9-15(26-12-34)3-6-18(21)24(29)33-25/h3-11H,1-2H3

InChI key

ZMGWMLCTFALXEN-UHFFFAOYSA-N

Application

Fluorescein diacetate 6-isothiocyanate (diacetyl-6-FITC, F6ITC), a structural analogue of fluorescein reactive with secondary amines, is used as an isomeric haptenic probe.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C A Swindlehurst et al.
Biophysical journal, 59(3), 619-628 (1991-03-11)
Relative differences in the active site environment of a monoclonal antibody when covalently bound to two isomeric haptens were studied using fluorescence quenching and lifetime measurements. Murine monoclonal antibody 4-4-20, a well-characterized high affinity antifluorescein antibody, served as the model
K M Weidner et al.
Molecular immunology, 29(3), 303-312 (1992-03-11)
Immunizations of high affinity anti-fluorescein monoclonal antibody 4-4-20 affinity labeled with fluorescein 5-isothiocyanate into a rabbit elicited antibodies specific for the liganded conformation of 4-4-20 (termed "anti-metatype" antibodies). Reaction of liganded 4-4-20 with anti-metatype antibodies caused significant delay (up to
Analysis of 3-(2-(ethylamino)propyl)-1,2,3,4-tetrahydro-5H(1)benzopyrano (3,4-c)pyridin-5-one in plasma by liquid chromatographic column switching after derivatizing the secondary amine with fluorescein-6-isothiocyanate.
Reynolds DL, Pachla LA.
J. Pharm. Pharm. Sci., 74, 1091-1094 (1985)

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