Protein crystallization is a major bottleneck in protein X-ray crystallography, the workhorse of most structural proteomics projects. Because the principles that govern protein crystallization are too poorly understood to allow them to be used in a strongly predictive sense, the
Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment. With 24-h-old competent cells we obtained routinely 2 . 10(7) transformants per microgram
Aerobic metabolism requires a complex antioxidative system to balance reactive oxygen species (ROS). When in excess, ROS disrupt cellular activities and molecular structures. Owing to the variety of ROS, there are different antioxidative activities that require various tests for their
Environmental science and pollution research international, 22(23), 18894-18905 (2015-07-25)
Irrigation with wastewater is a promising option to improve crop yields and to reduce pressure on freshwater sources. However, heavy metal concentrations in wastewater may cause health concerns. A greenhouse pot experiment was conducted in order to determine cadmium (Cd)
Journal of chromatography. A, 1360, 217-228 (2014-08-26)
Obtaining maximal sensitivity of nano UHPLC-MS/MS methods is primordial to quantify picomolar concentrations of neuropeptides in microdialysis samples. Since aspecific adsorption of peptides to Eppendorf tubes, pipette tips and UHPLC vials is detrimental for method sensitivity, a strategy is presented
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