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Sigma-Aldrich

Carbol-Fuchsin solution according to Kinyoun

for microscopy

Synonym(s):

Kinyoun’s carbol-Fuchsin

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About This Item

MDL number:
MFCD00143924
UNSPSC Code:
12171500
PubChem Substance ID:
329752209
NACRES:
MA.02

grade

for microscopy

Quality Level

100

form

liquid

composition

fuchsin basic (certified stain; C.I. 42500), 3 % (w/w)

impurities

phenol

color

red to very dark red

density

0.99 g/mL at 20 °C

ε (extinction coefficient)

77 at 546 nm in 50% ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

NC1=CC=C(C(C2=CC=C(N)C=C2)=C(C=C3)C=CC3=[NH2+])C=C1.[Cl-]

InChI

1S/C21H21N3/c1-13-11-16(5-9-19(13)23)21(15-3-7-18(22)8-4-15)17-6-10-20(24)14(2)12-17/h3-12,22H,23-24H2,1-2H3

InChI key

IMOKRVRFBCWWGH-UHFFFAOYSA-N

General description

Carbol-Fuchsin is an acid-fast stain that consists of basic fuchsin and phenol. It is a dry staining dye for staining acid-fast bacteria such as Mycobacterium tuberculosis. Carbol-Fuchsin solution according to Kinyoun is a modified stain that does not require heat to infuse the carbol-fuchsin into the acid-fast cells during the staining process, and this process is also known as “cold staining”. It requires a higher concentration of the primary stain.

Application

  • Kinyoun’s carbol-fuchsin is intended for use to stain paraffin sections of a formaldehyde-fixed specimen containing tubercle bacilli, and for the demonstration of tuberculosis bacteria (Mycobacterium tuberculosis).
  • It is also applicable for other organisms that retain the dye after immersion in acidified 70% alcohol.
  • It has been used to develop a staining technique for detecting microsporidial spores in fecal specimens.

Principle

Acid-fast bacteria possess a waxy, lipid-rich cell wall that absorbs dyes very slowly. To enhance the absorption of carbol-fuchsin dye and the subsequent formation of the mycolate-fuchsin complex, the dye solution applied to the specimen is normally heated to the evaporation point. In Kinyoun’s modification of this method, the heating of the solution is avoided. Consequently, hazardous phenolic vapors are not released.

Other Notes

Stain used for the demonstration of tuberculosis bacteria

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

109.9 °F - closed cup

Flash Point(C)

43.3 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM3860
BCCL4681
BCCL3237
BCCL7129
BCCK9547

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Fatmah M Salleh et al.
Diagnostic microbiology and infectious disease, 69(1), 82-85 (2010-12-15)
This study was conducted to evaluate the modification of the usual Gram-chromotrope staining technique developed in-house known as Gram-chromotrope Kinyoun (GCK) in comparison with the Weber Modified Trichrome (WMT) staining technique; as the reference technique. Two hundred and ninety fecal
L. Hallmann et al.
Klinische Mikrobiologie null
Ritu Singhal et al.
International journal of mycobacteriology, 4(1), 1-6 (2015-12-15)
Tuberculosis continues to cast a huge impact on humanity with its high incidence and mortality, especially in developing countries. For tuberculosis case detection, microscopy continues to be indispensible, given its low cost, rapidity, simplicity of procedure and high specificity. Modifications
Evaluation of gram-chromotrope kinyoun staining technique: its effectiveness in detecting microsporidial spores in fecal specimens
Salleh, et al.
Diagnostic Microbiology and Infectious Disease, 69, 82-85 (2011)

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