Skip to Content
Merck
All Photos(1)

Documents

11332465001

Sigma-Aldrich

TWEEN® 20

non-ionic, aqueous solution, 10% (w/v)

Synonym(s):

TWEEN 20, Tween, Tween 20

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352200

description

Formula: C58H114O26, for n = 20

form

solution

mol wt

Mr 1228 (for n= 20)

packaging

pkg of 5 × 10 mL

manufacturer/tradename

Roche

technique(s)

cell culture | plant: suitable
microbiological culture: suitable

CMC

~0.06 mM

absorption

= 0.025 at 254 nm at 0.05 % (w/v)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Tween 20, also referred to as poly(oxyethylene)n-sorbitan-monolaurate, is a non-ionic surfactant. It is mainly used in membrane research.

Application

Tween 20 is used for:
  • solubilizing membrane proteins during isolation of membrane-protein complexes.
  • a subsequent protein detection by UV light absorption, due to its low UV absorbance.
  • ELISA (enzyme-linked immunosorbent assay) and immunoblots.

Quality

Purity: <2 ppm peroxide content (as H2O2), <200 μM carbonyls, <100 μS conductivity. Specially purified for membrane research.

Physical form

10% (w/v) aqueous solution under nitrogen, supplied in injection glass bottles.

Preparation Note

Working concentration: >10 mg/mg membrane lipid

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Danny K Chou et al.
Journal of pharmaceutical sciences, 94(6), 1368-1381 (2005-04-29)
The objectives of this work were to determine the effects of nonionic surfactants (Tween 20 and Tween 80) on agitation-induced aggregation of the recombinant fusion protein, Albutropintrade mark (human growth hormone genetically fused to human albumin), and to characterize the

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service