10109134001
Roche
RNase
from bovine pancreas
Synonym(s):
Rnase
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About This Item
UNSPSC Code:
12352204
Pricing and availability is not currently available.
Recommended Products
biological source
bovine pancreas
Quality Level
form
dry powder (prepared from crystallized ribonuclease)
specific activity
~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).)
packaging
pkg of 500 mg
manufacturer/tradename
Roche
shipped in
wet ice
storage temp.
2-8°C
General description
Crude mixture of RNases.Dry powder (prepared from crystallized ribonuclease).
Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.[1]
Specificity
Heat inactivation: 1 mM EDTA
Application
RNase has been used in DNA ladder assay.[2]
Unit Definition
One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under the assay conditions; A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
Preparation Note
Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.
Working solution: It is recommended to dissolve the RNase (1 mg/ml) in water or TE buffer, pH 7.5.
Working solution: It is recommended to dissolve the RNase (1 mg/ml) in water or TE buffer, pH 7.5.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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