SCT117

BioTracker NIR694 Nuclear Dye (Water)

Live cell imaging far red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.

• Synonym(s): Live cell imaging probe
• UNSPSC Code: 12352207
• NACRES: NA.47

Properties

• technique(s): cell based assay: suitable
• detection method: fluorometric
• shipped in: ambient

Description

General description

The nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cell′s genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.

The BioTracker NIR694 Nuclear Dye (Water) is a far red nuclear stain for live cells. The dye can be excited by wavelengths from 488 to 647nm and emits far red fluorescence with emission maximum at 694nm. The dye specifically stains the nucleus of live cells. Staining does not require a wash step and demonstrates greater photostability than the traditional blue fluorescent nuclear stains DAPI and Hoechst. also can be used to stain live cells for cell cycle distribution analysis by flow cytometry BioTracker NIR694 Nuclear Dye (Water) staining is not fixable. For fixed cells we recommend the BioTracker NIR694 Nuclear Dye (DMSO).

Spectral Properties
Absorbance: 662nm
Emission: 694nm

Application

Live cell fluorescent imaging

Live cell imaging far red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.

Research Category
Cell Imaging

Research Sub Category
Live Cell Dye

Quality

Spectral Properties
Absorbance: 662nm
Emission: 694nm

Physical form

Liquid

Storage and Stability

Store BioTracker NIR694 Nuclear Dye (Water) at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): does not flash
• Flash Point(C): does not flash