antibody form
purified antibody
Quality Level
clone
NCM II 100, monoclonal
form
lyophilized
species reactivity
human, mouse
isotype
IgG1
General description
Purified mouse monoclonal antibody derived by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes N-myc as well as its cleavage products.
Recognizes N-myc as well as its cleavage products in IMR5 cells. Myc is a labile protein. Fresh lysates in the presence of protease inhibitor cocktails are recommended.
The v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. Amplification of the N-myc gene has been found in human neuroblastomas and cell lines. The extent of N-myc amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-myc gene gives rise to at least two nuclear phosphoproteins of ~64 kDa and ~67 kDa that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.
Immunogen
recombinant, human, N-Myc fusion protein, expressed in E. coli
Application
Frozen Sections (1-5 μg/ml)
Immunoblotting (1-5 μg/ml, see application references)
Immunofluorescence (1-5 μg/ml, see application references)
Immunoprecipitation (1-2 μg/sample, see application references)
Immunoblotting (1-5 μg/ml, see application references)
Immunofluorescence (1-5 μg/ml, see application references)
Immunoprecipitation (1-2 μg/sample, see application references)
Physical form
Lyophilized from a volatile buffer, 100 μg BSA.
Reconstitution
Reconstitute the lyophilized antibody with 1 ml sterile PBS. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-2
Analysis Note
Positive Control
IMR5 cells
IMR5 cells
Other Notes
It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal
Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
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The myc oncogene: its role in transformation and differentiation.
M D Cole
Annual review of genetics, 20, 361-384 (1986-01-01)
D J Slamon et al.
Science (New York, N.Y.), 232(4751), 768-772 (1986-05-09)
The human N-myc gene is related to the c-myc proto-oncogene, and has been shown to have transforming potential in vitro. Many studies have reported amplification of N-myc in human neuroblastoma and retinoblastoma cell lines. In primary tumors, amplification of the
W H Brondyk et al.
Oncogene, 6(7), 1269-1276 (1991-07-01)
The N-myc gene is amplified in several types of human tumors. To assess the role of the N-myc gene in the transformation of normal human cells, we transfected an N-myc expression vector into diploid human fibroblasts. Transfected clones were isolated
N Ikegaki et al.
Proceedings of the National Academy of Sciences of the United States of America, 83(16), 5929-5933 (1986-08-01)
Increased N-myc (now designated NMYC in human gene nomenclature) gene expression has been detected at the transcriptional level in certain types of neoplasms. As yet, the N-myc gene product has not been identified. To detect and characterize the N-myc gene
G M Brodeur et al.
Science (New York, N.Y.), 224(4653), 1121-1124 (1984-06-08)
A domain of DNA designated N-myc is amplified 20- to 140-fold in human neuroblastoma cell lines but not in cell lines from other tumor types. N-myc has now been found to be amplified in neuroblastoma tissue from 24 of 63
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