MHA000T2B

Tryptic Soy Broth- Ready-to-use Bags, Tubes & Ampoules

ampule of 2 mL, pack of 50 ampules, for use with MF-method, suitable for microbiology

• Synonym(s): CASO, Casein Soybean Digest Broth, Tryptone Soya Broth, SCDB, TSB
• UNSPSC Code: 41106225
• eCl@ss: 32160801
• NACRES: NA.74

Properties

• form: liquid
• Quality Level: 100
• shelf life: 12 mo.
• feature: ready-to-use
• packaging: ampule of 2 mL; pack of 50 ampules
• technique(s): cell counting: suitable (total viable count (TVC))
• color: light amber
• input: sample type bottled water; sample type cosmetics; sample type beverage(s); sample type food(s); sample type wine; sample type sport drink; sample type cider; sample type beer; sample type soft drink
• pH: 7.3±0.2 (25.0 °C)
• application(s): clinical; cosmetics; environmental; food and beverages; industrial qc; microbiology
• compatibility: for use with MF-method; for use with Microfil^®; for use with MicropreSure monitor; for use with MicropreSure^®; for use with Milliflex^®
• shipped in: wet ice
• storage temp.: 2-8°C
• suitability: nonselective for

Description

General description

Tryptic Soy Broth is a general purpose liquid enrichment medium used in qualitative procedures for the sterility test and for the enrichment and cultivation of aerobic and facultatively anaerobic microorganisms that are not excessively fastidious.{18} In clinical microbiology, it may be used for the suspension, enrichment and cultivation of strains isolated on other media. In clinical microbiology, the medium is used in a variety of procedures, e.g., for the preparation of the inoculum and for suspending strains for Kirby-Bauer disc diffusion susceptibility testing and for the microbiological test procedure of culture media. Enzymatic digests of casein and soybean provide amino acids and other complex nitrogenous substances. Glucose (=dextrose) is an energy source. Sodium chloride maintains the osmotic equilibrium. Dibasic potassium phosphate acts as a buffer to control pH. Colonies appear clear to white colonies; some may produce pigment.

Application

Tryptone Soy Broth is used as a non-selective medium used to detect total heterotrophic microorganisms in water, dairy products and other liquids like Beer, Bottled Water, Cider, Soft Drink, Sport Drink and Wine. The media finds its application in Clinical, Cosmetics, Environmental Analysis, Food and Beverage and Industrial QC.

Packaging

2 mL plastic ampoules

Specifications

Incubation Temperature: 25–35 °C

Incubation Time: 48–72 hrs

Culture Medium

Trypticase Soy

Analysis Note

Clear to white colonies; some may produce pigment.

QC organisms
S. epidermidis (ATCC 14990)

C. albicans (ATCC 10231)

E. coli (ATCC 25922)

B. subtilis (ATCC 6633)

A.brasilliensisformerly A. niger (ATCC 16404 / WDCM 00053)

P. aeruginosa (ATCC 27853)

Target Microorganisms: Total Count

Legal Information

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable