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MABT400

Sigma-Aldrich

Anti-Lubricin/Proteoglycan 4, clone 5C11 Antibody

clone 5C11, from mouse

Synonym(s):

Proteoglycan 4, Lubricin, Megakaryocyte-stimulating factor, Superficial zone proteoglycan, Proteoglycan 4 C-terminal part

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

5C11, monoclonal

species reactivity

canine, bovine, goat, rat, porcine, human, guinea pig

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001121180

UniProt accession no.

Q92954

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PRG4(10216)

General description

The protein called Lubricin/Proteoglycan 4 is an important secreted protein in the joint critical for joint function and thus is also a focus of research in joint diseases as well. Lubricin/Proteoglycan 4 plays a role in boundary lubrication and prevents the deposition of cartilage from the synovial fluid by controlling the synovial growth of the synovial cells and inhibiting the adhesion of the cells to the cartilage surface. During development, Lubricin/Proteoglycan 4 also acts as a growth factor for hematopoietic and endothelial cell lineages, and Lubricin/Proteoglycan 4 appears important for proper barrier and lubrication development in tissues like the eye. Lubricin/Proteoglycan 4 is highly expressed in joints and near joint boundaries but can also be found in the brain, heart, lung, kidney and other tissues. Lubricin/Proteoglycan 4 has several isoforms that differ in their cell and tissue expression. Isoform C and D are the most widely expressed forms. Finally because of its effects on surfaces, Lubricin/Proteoglycan 4 is being examined in tissue engineering and artificial cartilage development as a treatment for joint diseases.

Immunogen

Recombinant protein corresponding to human Lubricin/Proteoglycan 4.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lubricin/Proteoglycan 4 in human liver and human heart tissue.
Western Blotting Analysis: A representative lot detected purified human synovial lubricin, as well as lubricin in synovial fluid recovered from patients with osteoarthritis (OA), camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), or from bovine, porcine, goat, dog, rat, and guinea pig (Ai, M., et al. (2015). PLoS One. 10(2):e0116237).
Western Blotting Analysis: A representative lot detected purified bovine synovial lubricin from calf and steer under both reduced and non-reduced conditions (Schmidt, T.A., et al. (2009). Biochim Biophys Acta. 1790(5):375-384).
This Anti-Lubricin/Proteoglycan 4, clone 5C11 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Lubricin/Proteoglycan 4.

Quality

Evaluated by Western Blotting in Lubercin purified protein.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected 10 µg of purified Lubricin/Proteoglycan 4 protein.

Target description

~220 kDa observed. The calculated molecular weight of this protein is 151 kDa with many isoforms observed around 145 kDa and it can run higher due to glycosylation.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Patrick Weber et al.
Advanced healthcare materials, e2401623-e2401623 (2024-07-15)
Osteoarthritis is a degenerative joint disease that is associated with decreased synovial fluid viscosity and increased cartilage friction. Though viscosupplements are available for decades, their clinical efficacy is limited and there is ample need for more effective joint lubricants. This
Taryn E Ludwig et al.
BMC musculoskeletal disorders, 16, 386-386 (2015-12-17)
Proteoglycan 4 (PRG4) and hyaluronan (HA) are key synovial fluid constituents that contribute synergistically to cartilage boundary lubrication; however, the effects of their concentrations as well as their structure, both of which can be altered in osteoarthritis, on this functional
A single-cell comparison of adult and fetal human epicardium defines the age-associated changes in epicardial activity.
Knight-Schrijver, et al.
Nature cardiovascular research, 1, 1215-1229 (2023)
Dongrim Seol et al.
Human gene therapy, 33(9-10), 529-540 (2021-10-07)
Lubricin, a glycoprotein encoded by the proteoglycan 4 (PRG4) gene, is an essential boundary lubricant that reduces friction between articular cartilage surfaces. The loss of lubricin subsequent to joint injury plays a role in the pathogenesis of posttraumatic osteoarthritis. In
Joseph A M Steele et al.
Biomaterials, 286, 121548-121548 (2022-05-20)
Articular cartilage is comprised of zones that vary in architecture, extracellular matrix composition, and mechanical properties. Here, we designed and engineered a porous zonal microstructured scaffold from a single biocompatible polymer (poly [ϵ-caprolactone]) using multiple fabrication strategies: electrospinning, spherical porogen
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