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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
46, monoclonal
mol wt
calculated mol wt 45.05 kDa
observed mol wt ~45 kDa
species reactivity
virus
packaging
antibody small pack of 100 μL
technique(s)
ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
UniProt accession no.
shipped in
dry ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
Unknown ... N nucleocapsid protein(14254601)
General description
Nucleoprotein (UniProt: R9UM87; also known as Nucleocapsid protein, NC, Protein N) is encoded by the N gene (Gene ID: 14254601) in Middle East respiratory syndrome-related coronavirus (MERS-CoV). The MERS-CoV is a positive-strand RNA virus of the betacoronavirus genus within the Coronaviridae family that causes severe respiratory syndrome in human. The mature MERS-CoV contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). Nucleoprotein of MERS-CoV is a homooligomeric multifunctional protein that plays roles in genome encapsidation, RNA synthesis during replication, host translational shutoff, and innate immunity disruption. It packages the positive strand viral genome RNA into helical ribonucleocapsid and is involved in virion assembly through its interactions with the viral genome and membrane protein M. The nucleoprotein is reportedly organized into two folded domains known as the N-terminal domain (NTD) and the C-terminal domain (CTD) that are separated by a disordered region (LKR) containing a serine/ arginine stretch. Clone 46 displays specificity for MERS-CoV nucleoprotein and does not show cross-reactivity with nucleoprotein from other related viruses, including SARS coronavirus. (Ref.: Nguyen, THV., et al. (2019). Acta Crystallogr. D. Struct. Biol. 75(1); 8-15; Yamaoka, Y., et al. (2016). Front. Microbiol. 7; Article 509).
Specificity
Clone 46 is a mouse monoclonal antibody that detects nucleoprotein from Middle East respiratory syndrome-related coronavirus (MERS-CoV).
Immunogen
His-tagged recombinant fragment corresponding to 292 amino acids from the nucleoprotein of Middle East respiratory syndrome-related coronavirus (MERS-CoV).
Application
Quality Control Testing
Evaluated by Western Blotting with recombinant MERS-CoV nucleoprotein.
Western Blotting Analysis: A 1:50,000 dilution of this antibody detected recombinant MERS-CoV Nucleoprotein.
Tested Applications
ELISA Analysis: A representative lot detected MERS-CoV Nucleoprotein in ELISA applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).
Western Blotting Analysis: A representative lot detected MERS-CoV Nucleoprotein in Western Blotting applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).
Immunoprecipitation Analysis: A representative lot immunoprecipitated MERS-CoV Nucleoprotein in Immunoprecipitation applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting with recombinant MERS-CoV nucleoprotein.
Western Blotting Analysis: A 1:50,000 dilution of this antibody detected recombinant MERS-CoV Nucleoprotein.
Tested Applications
ELISA Analysis: A representative lot detected MERS-CoV Nucleoprotein in ELISA applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).
Western Blotting Analysis: A representative lot detected MERS-CoV Nucleoprotein in Western Blotting applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).
Immunoprecipitation Analysis: A representative lot immunoprecipitated MERS-CoV Nucleoprotein in Immunoprecipitation applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-MERS-CoV Nucleoprotein, clone 46, Cat. No. MABF3048, is a mouse monoclonal antibody that detects MERS-CoV Nucleoprotein and is tested for use in ELISA, Immunoprecipitation, and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at +2°C to +8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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