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CP28

Sigma-Aldrich

Anti-Fibroblast Antigen Mouse mAb (AS02)

liquid, clone AS02, Calbiochem®

Synonym(s):

Anti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

AS02, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, monkey

should not react with

rat, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... FGF22(27006)

General description

Anti-Fibroblast Antigen, mouse monoclonal, clone AS02, detects ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts (non-reducing conditions). It is validated for FC, WB, IF, IP & IHC (frozen sections).
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with X63-Ag.8.653 mouse myeloma cells. Recognizes the ~30-31 kDa fibroblast antigen (CD90/Thy-1) under non-reducing conditions.
Recognizes the ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts under non-reducing conditions.

Immunogen

Human
human fibroblasts

Application

Flow Cytometry (see comments)

Frozen Sections (10 µg/ml, acetone fixation)

Immunoblotting (10 µg/ml, non-reducing only)

Immunofluorescence (10 µg/ml)

Immunoprecipitation (2-10 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM sodium phosphate buffer, 6% BSA.

Analysis Note

Positive Control
Any fibroblast cell line or skin tissue

Other Notes

Saalbach, A., et al. 1996 J. Invest. Dermatol.106, 1314.
Janin, A., et al. 1990. Clin. Exp. Rheum.8, 237.
Kay, M., et al. 1989. J. Invest. Dermatol.92, 168.
Esterre, P.H., et al. 1984 Cell Mol. Biol.38, 297.
Hoyhtya, M., et al. 1984 Eur. J. Biochem141, 477.
This antibody has been used with magnetic beads to remove fibroblasts from mixed cultures. It is also suitable for flow cytometry studies (see application references). Note that in healthy tissue (skin, placenta, liver, heart), this antibody does not react with endothelial cells. However, endothelial cells activated by PMA, TNFα, or IFNγ may be detected by this antibody, suggesting activated endothelial cells may express CD90 and that CD90 may be an activation marker of human endothelial cells. Antibody should be titrated for optimal results in individual systems.

Legal Information

Manufactured by Dianova, GmbH, Germany.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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