CC130

Human Vitronectin

recombinant, expressed in E. coli, liquid, 0.5 mg/mL, used as an effective xeno-free alternative to matrigel, suitable for human ES and iPS cell culture, PLURISTEM^®

• Synonym(s): S-protein, Serum-spreading factor, V75
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

Properties

• product name: PluriSTEM-XF^® Recombinant Vitronectin, Human pluripotent stem cell culture.
• biological source: Escherichia coli; human
• Quality Level: 100
• Assay: ≥92% (SDS-PAGE)
• form: liquid
• manufacturer/tradename: PluriSTEM^®
• concentration: 0.5 mg/mL
• technique(s): cell attachment: suitable; cell culture | mammalian: suitable; cell culture | stem cell: suitable
• input: sample type: human embryonic stem cell(s)
• UniProt accession no.: P04004
• shipped in: dry ice

Description

General description

PluriSTEM Recombinant Vitronectin is an effective xeno-free alternative to matrigel and has been validated to successully culture human ES and iPS cells using PluriSTEM Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.

PluriSTEM Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.

Application

Research Category
Stem Cell Research

Cell Structure

ECM & Adhesion

Supports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.

Quality

Each lot is tested on human ES cells (H1) cultured using 5-10 μg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate).

Physical form

Product is sterile-filtered in a 20 mM pH 8.0 Tris-HCl buffer, with a proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and glycerol.

Storage and Stability

Store at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles.

Legal Information

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable