Dopamine Beta Hydroxylase (DbetaH) C-terminal. By immunoblot the antibody specifically reacts with a single (or double) band(s) of M.W. »70-75,000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE.
Immunogen
Epitope: C-terminus
Synthetic peptide, CPTSQGRSPAGPTVVSI-amide, cloned from human (DbH) cDNA (Nagatsu et al. 1990).
Application
Anti-Dopamine β Hydroxylase Antibody, C-terminus is an antibody against Dopamine β Hydroxylase for use in IH & WB.
Immunoblotting: 1:250-1:1,000.
Immunohistochemistry: 1:250-1:1,000, staining was evaluated in the locus coeruleus of formaldehyde-fixed, cryopreserved human and monkey brain. Showed moderate staining on both human and monkey.
Optimal working dilutions must be determined by the end user.
Research Category Neuroscience
Research Sub Category Neurotransmitters & Receptors
Neuronal & Glial Markers
Physical form
Liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Storage and Stability
Maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze-thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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