345806-M

β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli

• Synonym(s): β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli, β1,4-D-Galactoside Galactohydrolase
• CAS Number: 9031-11-2
• Enzyme Commission number: 3.2.1.23 (BRENDA, IUBMB)
• MDL number: MFCD00036918
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• form: liquid
• specific activity: ≥1.5 units/mL; ≥6 units/mg protein
• packaging: pkg of 50 mIU
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• foreign activity: N-acetylglucosaminidase, α-galactosidase, α-mannosidase, neuraminidases, proteases, none detected
• storage temp.: 2-8°C

Description

General description

Note: 1 mU = 1 milliunit.

Recombinant, Streptococcus pneumoniae β1,4-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,4-linked galactose. This enzyme is not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.

Application

Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5. Sterile solution.

Other Notes

Kierman, U.A., et al. 2004. Proteomincs4, 1825.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable