797006
In Vitro Protein Expression (iPE-SS) Kit for disulfide-containing proteins
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.12
Pricing and availability is not currently available.
Recommended Products
usage
Stable Isotope Labeling
1 extractions
Quality Level
technique(s)
bio NMR: suitable
protein expression: suitable
storage temp.
−70°C
Application
This is a kit which is improved for the synthesis of extracellular secretory proteins such as cytokines and antibodies. This kit is suitable for the synthesis of stable isotope labeled disulfide-containing proteins.
This kit has been developed under license from RIKEN, incorporating their proprietary, advanced cell-free protein synthesizing technology into a kit dedicated to stable isotope labeling.
Packaging
Stable isotope labeled amino acid mixtures are not included in this kit. Please use the separately sold stable isotope labeled amino acids mixtures. (Prod. No. 767972, 767964, and 771031)
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
10 - Combustible liquids
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Jun Yokoyama et al.
Analytical biochemistry, 411(2), 223-229 (2011-01-25)
During recent years, the targets of protein structure analysis using nuclear magnetic resonance spectroscopy have become larger and more complicated. As a result, a complete and precise stable isotope labeling technique has been desired. A cell-free protein synthesis system is
Takayoshi Matsuda et al.
Biochemical and biophysical research communications, 431(2), 296-301 (2013-01-08)
Many important therapeutic targets are secreted proteins with multiple disulfide bonds, such as antibodies, cytokines, hormones, and proteases. The preparation of these proteins for structural and functional analyses using cell-based expression systems still suffers from several issues, such as inefficiency
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