486825
L-Leucine-5,5,5-d3
99 atom % D
Synonym(s):
SILAC Amino Acid
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About This Item
Linear Formula:
CH3CH(CD3)CH2CH(NH2)CO2H
CAS Number:
Molecular Weight:
134.19
MDL number:
UNSPSC Code:
12352209
PubChem Substance ID:
NACRES:
NA.12
Pricing and availability is not currently available.
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isotopic purity
99 atom % D
Quality Level
Assay
99% (CP)
form
solid
optical activity
[α]25/D +14.5°, c = 2 in 5 M HCl
technique(s)
protein expression: suitable
mp
>300 °C (lit.)
mass shift
M+3
SMILES string
[2H]C([2H])([2H])C(C)C[C@H](N)C(O)=O
InChI
1S/C6H13NO2/c1-4(2)3-5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t5-/m0/s1/i1D3/t4?,5-
InChI key
ROHFNLRQFUQHCH-LONBSJBQSA-N
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General description
L-leucine-5,5,5-d3 is an isotopic analogue of L-leucine.
Application
L-leucine-5,5,5-d3 is used in the quantitative determination of proteins using mass spectrometry and also used in the synthesis of (2S,4R)-(5,5,5-d3)leucine via stereospecific manner.
Used in the methodology of stable isotope tagging of epitopes (SITE) for identification of virus-induced peptides in vaccine development.
Packaging
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Gerald W Becker
Briefings in functional genomics & proteomics, 7(5), 371-382 (2008-12-25)
Straightforward methods for the introduction of stable isotopes into proteins with subsequent isolation and purification of the proteins will greatly aid the field of quantitative proteomics. Proteins containing amino acids with one or more of the stable isotopes of deuterium
Hugo D Meiring et al.
Molecular & cellular proteomics : MCP, 5(5), 902-913 (2006-01-25)
Identification of peptides presented in major histocompatibility complex (MHC) class I molecules after viral infection is of strategic importance for vaccine development. Until recently, mass spectrometric identification of virus-induced peptides was based on comparative analysis of peptide pools isolated from
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