ZRB2668

Anti-Ovalbumin Antibody, clone 3G13 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Allergen Gal d II, Egg albumin, Plakalbumin
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3G13, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 42.88 kDa; observed mol wt ~43 kDa
• purified by: using Protein A
• species reactivity: chicken
• packaging: antibody small pack of 25
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): ELISA: suitable; affinity binding assay: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Unknown
• Protein ID accession no.: NP_990483
• UniProt accession no.: P01012
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: chicken ... SERPINB14(396058)

Description

General description

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 3G13 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Ovalbumin.

Immunogen

His-tagged full-length, recombinant Ovalbumin from Chicken egg.

Application

Quality Control Testing

Evaluated by Western Blotting in Chicken egg ovalbumin.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Chicken egg ovalbumin.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected recombinant Chicken egg Ovalbumin..

Affinity Binding Assay Analysis: A representative lot of this antibody bound Ovalbumin with a KD of 2.6 x 10-7 in an affinity binding assay.

Enzyme Immunoassay (ELISA) Analysis: Serial of dilutions from a representative lot detected Ovalbumin.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Ovalbumin (UniProt: P01012; also known as Allergen Gal d II, Egg albumin, Plakalbumin, Gal d 2) is encoded by the SERPINB14 gene (Gene ID: 396058) in chicken. Ovalbumin is a member of the serine protein inhibitor (serpin) super family, but it considered as a non-inhibitory serpin. It is a storage protein of egg white and may make up about 60% of the total protein in egg white. Ovalbumin provides a major interest for study because its structure provides a model for the structure of the inhibitory serpins. It is known to chelate heavy metals and traps the metal ions within the sulfhydryl bonds of the protein, which prevents the absorption of the metals into the gastrointestinal tract and prevents poisoning. Ovalbumin is a monomeric phosphoglyco-protein with an isoelectric point of 4.5. It is the only egg protein that contains free sulfhydryl groups. The purified form of ovalbumin consists of three different subclasses, Al, A2 and A3, which contain two, one and no phosphate groups per molecule, respectively. During storage of fertilized and non-fertilized eggs or under alkaline conditions, the native ovalbumin (N-ovalbumin) is transformed into a thermostabilized conformer, S-ovalbumin. In freshly laid eggs, only about 5% of ovalbumin exists in the S-form, while more than 50% of the ovalbumin converts to the S species during storage. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mine, Y., et al. (1991). J. Agric. Food Chem. 39(3); 443-446).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable