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Key Documents

SRP2016

Sigma-Aldrich

GAL4 [(1-147) - AH] from Saccharomyces cerevisiae

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym(s):

Gal81

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.26

biological source

Saccharomyces cerevisiae

recombinant

expressed in E. coli

Assay

≥80% (SDS-PAGE)

form

frozen liquid

mol wt

~18.6 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

300 μg/mL

technique(s)

electrophoretic mobility shift assay: suitable

color

colorless to clear

NCBI accession no.

NM_001184062

UniProt accession no.

P04386

shipped in

dry ice

storage temp.

−70°C

Gene Information

Saccharomyces cerevisiae ... GAL4(855828)

Biochem/physiol Actions

Transcriptional activity is greatly stimulated by promoter-specific activator proteins. These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain. The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5′-CGGN5(T/A)N5CCG-3′ (5 ). A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. Linking of an acidic synthetic peptide, forming an α-helix (AH), to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure. This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J Ma et al.
Cell, 48(5), 847-853 (1987-03-13)
We describe the activities of a wide array of deletion mutants of GAL4, a yeast transcriptional activator. We identify two short regions of GAL4, each of which activates transcription when fused to the DNA-binding region of the molecule. Very large
M Ptashne et al.
Nature, 346(6282), 329-331 (1990-07-26)
Proteins that activate genes are quite disparate in character; in particular, some work 'universally' and others do not. A simple model can accommodate most of the recently published results.
Commitment and activation at pol II promoters: a tail of protein-protein interactions.
B Lewin
Cell, 61(7), 1161-1164 (1990-06-29)

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