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SRP0213

Sigma-Aldrich

PTP1B (1-321)/PTPN1 Active from mouse

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym(s):

PTPN1, Protein tyrosine phosphatase, non-receptor type 1

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

mouse

recombinant

expressed in E. coli

Assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

63.3 kDa

packaging

pkg of 20 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PTPN1(5770)

General description

Protein tyrosine phosphatase, Catalytic domain, (amino acids 1-321), with N-terminal GST tag, MW = 63.3 kDa, expressed in an E. coli expression system.

Application

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physical form

Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 10 mM glutathione, 1 mM DTT and 50% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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