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Key Documents

SRE0021

Sigma-Aldrich

Granzyme B human

recombinant, expressed in insect cells, ≥90% (SDS-PAGE)

Synonym(s):

Granzyme B human, Cytotoxic T-lymphocyte proteinase 2, Granzyme-2, GranzymeB, Lymphocyte protease, SECT, T-cell serine protease 1-3E

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.32

biological source

human

Quality Level

200

recombinant

expressed in insect cells

Assay

≥90% (SDS-PAGE)

form

lyophilized powder

potency

>0.004 units/mg

specific activity

≥40 units/μg

packaging

pkg of 5 μg

UniProt accession no.

P10144

shipped in

wet ice

storage temp.

−70°C

Gene Information

human ... GZMB(3002)

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General description

Granzyme B (GzmB) is a serine protease which is stored in granules of activated cytotoxic T cells and NK cells. GZMB codes for granzyme B.

Biochem/physiol Actions

Granzyme B is considered as the marker of activated CTLs (cytotoxic T-cells), which mediates target-cell apoptosis. It induces apoptosis in caspase-dependent and caspase-independent manner. After contact with target cells, granzyme B is directionally exocytosed, and then enters the target cell with assistance from perforin. Granzyme B specifically cleaves after Asp residues, and particularly processes and activates various pro-caspases. This induces apoptosis in the target cell.

Unit Definition

One unit is defined as the amount of enzyme that hydrolyzes 1 pmol/min of IETD-AFC per min at 37°C in 50 mM HEPES, pH 7.5 containing 10 mM CaCl2.

Physical form

Lyophilized powder, containing HEPES buffer (original solution at pH 7.5)

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Granzyme B enters the mitochondria in a Sam50-, Tim22-and mtHsp70-dependent manner to induce apoptosis
Chiusolo V, et al.
Cell Death and Differentiation, 24(4), 747-747 (2017)
Risk of generalized vitiligo is associated with the common 55R-94A-247H variant haplotype of GZMB (encoding granzyme B).
Ferrara TM, et al.
The Journal of Investigative Dermatology, 133(6), 1677-1677 (2013)

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