SAE0150

Chondroitinase ABC from Proteus vulgaris

recombinant, low endotoxin, aqueous solution, ≥100 U/ml, 50-250 units/mg protein, BSA free

• Synonym(s): Ch′ase ABC, Chondroitin ABC Lyase
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: bacterial (Proteus vulgaris)
• Quality Level: 200
• conjugate: (Glucosaminoglycan)
• specific activity: 50-250 units/mg protein
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Chondroitinase ABC is a lyase with specificity toward chondroitin sulfates and dermatan sulfates - with some minor activity toward hyaluronan. This recombinant version only contains the endolytic chondroitinase, meaning it will not digest the non-reducing ends of proteoglycan substrates. It targets β-(1,4)-linkages between N-acetylgalactosamine and either D-glucuronic acid or L-iduronic acid. The product of digestion is a mixture of Δ 4-unsaturated tetra- and di-saccharides.

Application

• Degradation of Chondroitin sulfate-containing tissue such as extracellular matrices and cartilage
• Neural regeneration research
• Eliminative degradation of all forms of Chondroitin sulfate: A, B and C
• Quantitation of glucosaminoglycans (mucopolysaccharides)

Features and Benefits

• Low Endotoxin
• BSA Free
• Recombinant
• Endolytic component only, does not contain exolytic enzyme.
• Compatible with chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate, and acts slowly on hyaluronate

Components

Non active Ingredients: Hepes-Sodium salt, NaCl, Trehalose, Imidazole, Glycerol, Water

Compatibility

pH optimum:

• pH 8.0 (chondroitin sulfate)
• pH 6.8 (hyaluronic acid)

Temperature optimum: 37 °C
Activator: 0.05 M acetate 
Inhibitors: 1 mM Zn²⁺ Ni²⁺, Fe²⁺ and Cu²⁺ 

Unit Definition

1 unit will liberate 1.0 μmole of unsaturated disaccharides per min at pH 8.0 at 37 °C, using chondroitin sulfate from shark cartilage (C4384) as substrate.

Storage and Stability

Store the product at -70 °C. When stored unopened at -70 °C, the buffer is stable for at least two years.

Other Notes

This product is for R&D use only. Not for drug, household, or other uses. Please consult the SafetyData Sheet for information regarding hazards and safe handling practices.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2