SAE0076

Tau-441 human

recombinant, lyophilized powder, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: calculated mol wt 50.3 kDa (including c-terminal His and V5 tags); observed mol wt 70-100 kDa by SDS-PAGE (The protein migrates as a 70-110 kDa protein on SDS-PAGE due to glycosylation)
• technique(s): cell based assay: suitable
• UniProt accession no.: P10636-8
• shipped in: ambient
• storage temp.: −20°C
• Gene Information: human ... MAPT(4137)

Description

General description

Research area: Neuroscience

Tau-441 is a member of the Tau family of proteins. Tau is a family of neuronal microtubule-associated proteins. Six isoforms have been found that differ from each other in having either 3 or 4 binding repeats (R) of 31-32 amino acids, and from zero to 3 amino terminal inserts (N) of 29 amino acids each.

Application

Tau-441 human has been used to study the tau uptake by human bronchial epithelial cells (HBEC).

Biochem/physiol Actions

Tau proteins are mainly expressed in the neurons of the central nervous system where they exert a role in stabilizing microtubules, key components of axonal transport, as well as in signal transduction. Tau proteins are subject to phosphorylation, which regulates their association with the microtubules. Deposits of Alzheimer′s disease AD-associated proteins, such as hyperphosphorylated Tau, as well as other shared misfolded proteins, such as beta-amyloid precursor protein (beta-APP), ubiquitin, and various chaperones and protein kinases, are thought to play a pathologic role in the cognitive decline and muscular failure. Malfunctioning of Tau proteins is associated with microtubule disintegration and collapsing of the neuronal transport system. Among other diseases, Tau forms in cerebrospinal fluid are considered a reliable biomarker for progressive supranuclear palsy, where the levels of Tau forms ratio were significantly reduced.

Sequence

MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGLDRIRGRKLGPFEGKPIPNPLLGLDSTRTGHHHHHHHHGGQ

This sequence includes a polyhistidine and V5 tags at the C-terminus.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable