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SAE0020

Sigma-Aldrich

Cellulase, enzyme blend

Synonym(s):

Cellic CTec2

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

form

aqueous solution

Quality Level

200

density

~1.15 g/mL

shipped in

ambient

storage temp.

2-8°C

General description

Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes.[1][2]
This product contains cellulases, ß-glucosidases, and hemicellulase, for the application of degrading cellulose to fermentable sugars. This product is effective on a wide variety of pre-treated lignocellulosic bimass materials, for converting the carbohydrates in these materials into simple sugars prior to fermentation, for application in biofuels research.

Application

Cellulase, enzyme blend has been used for the purpose of enzymatic hydrolysis.[3]

Biochem/physiol Actions

Cellulase is responsible for the breakdown of insoluble plant polymer cellulosic substrates into soluble sugars, mostly cellobiose and glucose.[1][2] It specifically catalyzes the hydrolysis of β -1,4 glucosidic bonds in cellulose.[2] Animals do not naturally possess the ability to digest cellulose, but do so with the help of gut microorganisms.[4]

Legal Information

A product of Novozyme Corp

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000317729
0000295048
0000295048
0000317729
0000292846

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A cellulase gene of termite origin.
Watanabe H, et al.
Nature, 394(6691), 330-330 (1998)
Liwen He et al.
Bioresource technology, 289, 121693-121693 (2019-07-02)
The optimum condition of steam explosion pretreatment was screened for hippophae, and anaerobic calcium oxide (CaO) alkalization was further used to improve its enzymatic hydrolysis. Steam-exploded hippophae reached the lowest pH value (4.01) and the maximal hemicellulose removal (77.16%) at
Zhe Ling et al.
Bioresource technology, 305, 123025-123025 (2020-03-03)
Discovering green solvents and their inner mechanisms for efficient deconstruction of lignocellulosic biomass recalcitrance are receiving growing interests. In this work, eco-friendly levulinic acid (LA) based deep eutectic solvents (DES) were proposed for pretreatment on moso bamboo by combining acetamide
Qiulu Chu et al.
Biotechnology for biofuels, 14(1), 136-136 (2021-06-14)
Ethanol organosolv (EOS) pretreatment is one of the most efficient methods for boosting biomass saccharification as it can achieve an efficient fractionation of three major constituents in lignocellulose. However, lignin repolymerization often occurs in acid EOS pretreatment, which impairs subsequent
Model of acetic acid-affected growth and poly (3-hydroxybutyrate) production by Cupriavidus necator DSM 545.
Marudkla J, et al.
Journal of Biotechnology, 268, 12-20 (2018)
View All Related Papers

Questions

1–8 of 8 Questions  

  1. In what kind of media/buffer does this blend work at its best ? Does it work well in EtOH ? Thanks

    1 answer

    1. The optimal activity for this blend is between pH 5 and 5.5. The Buffer Reference Center provides examples of buffers that work in this pH range.

      Helpful?

  2. Is this enzyme blend compatible with the feruloyl esterase enzyme ?

    1 answer

    1. This product contains cellulases, beta-glucosidases, and hemicellulase for degrading cellulose to fermentable sugars. It is effective on a wide variety of pre-treated lignocellulosic biomass materials, converting carbohydrates into simple sugars prior to fermentation, applicable in biofuels research. The compatibility of this enzyme blend with feruloyl esterase (ferulic acid esterase; a hemicellulase) was not evaluated in-house. However, published literature indicates that ferulic acid esterase pretreatment facilitates the cellulase hydrolysis process by synergistically opening the crosslinked network structures of cell walls with other cellulases and hemicellulases. For example, see the articles by Zhao, M. et al., Carbohydrate Polymers, 313, 120885 (2023) and Xu, Z., et al., Front. Microbiol., Sec. Microbial Physiology and Metabolism, 11, 568716 (2020).

      Helpful?

  3. What is the recommended amount of Cellic CTec2 enzyme for saccharification of 1 gram cellulose?

    1 answer

    1. Recommended enzyme trial dosages for Cellic CTec2 are 1.5%, 3.0%, 6.0%, and 30.0% weight/weight (grams enzyme per gram cellulose) for initial investigations. Further testing is necessary to refine the dose-response curve and assess the impact of time, solids loading, cellulose conversion, and enzyme dosage.

      Helpful?

  4. what is the concentration (units/ml) of the enzyme?

    1 answer

    1. This Cellulase blend is a product of Novozymes. The analytical testing confirms that this material meets the 1000 unit/gram minimum activity, as well as the lot specific density. The density range is 1.0 - 1.3 g/mL. As an example, a 50 mL package size for a lot reporting a density of 1.2 g/mL with an activity of 1000 units per gram would have a minimum concentration of 1200 u/mL. Please see the link below to access a sample or lot specific Certificate of Analysis:
      https://www.sigmaaldrich.com/product/sigma/sae0020#product-documentation

      Helpful?

  5. 1. How much glucose and xylose sugars are present in Cellic CTec2? 2. And how many other ingredients are included? 3. From what microorganisms do the Cellulase, β-glucosidase, and Hemicellulase enzymes originate?

    1 answer

    1. The composition of this material is considered proprietary. This product is manufactured by fermentation of microorganisms not present in the final product. Please see the link below to a publication that may be helpful.
      https://dspace.lib.cranfield.ac.uk/bitstream/handle/1826/15255/Augmented_hydrolysis_of_acid_pretreated_sugarcane_bagasse-2020.pdf;jsessionid=75390AF54DD520E2E7334DC8525A57C3?sequence=4

      Helpful?

  6. What is this cellulase enzyme activity? ?FPU

    1 answer

    1. This is a product of Novoxymes. The manufacturer notes that this cellulase will hydrolyze (1,4)-beta-D-glucoside linkages and reports the activity in Biomass hydrolysis Units or BHU-2 per gram. This activity is a minimum of 1000 BHU-2 units per gram.

      Helpful?

  7. OPTIMIZATION VALUES OF PH, T°, AND UNITS TO WORK WITH THIS ENZYME

    1 answer

    1. The optimum temperature is 45-50°C and the optimum pH is 5.0–5.5.

      Helpful?

  8. is it possible to know the MW for the enzymes

    1 answer

    1. This product is a proprietary blend of cellulases, ß-glucosidases, and hemicellulase. The molecular weight for this item is not determined.

      Helpful?

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