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SAB4200774

Sigma-Aldrich

Anti-Shiga Toxin 1, B Subunit (STxB) antibody, Mouse monoclonal

clone 13C4, purified from hybridoma cell culture

Synonym(s):

Anti-SLT-1 B subunit, Anti-SLT-1b, Anti-Shiga-like toxin 1 subunit B, Anti-Verocytotoxin 1 subunit B, Anti-Verotoxin 1 subunit B, Anti-stxB

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

13C4, monoclonal

description

Research area: Microbiome

form

buffered aqueous solution

mol wt

~7 kDa

species reactivity

E. coli

concentration

~1.0 mg/mL

technique(s)

flow cytometry: 2.5-5 μg/test using human RAMOS cells pretreated with recombinant Shiga toxin 1, B subunit
immunoblotting: 1-2 μg/mL using purified recombinant Shiga Toxin 1-B subunit produced in E. coli (using PVDF membrane)
immunoprecipitation (IP): suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal Anti-Shiga Toxin 1, B Subunit (mouse IgG1 isotype) is derived from the 13C4 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a purified Shiga-like toxin from E. coli H30. Shiga toxins consist of two polypeptides, an A chain and a B chain non-covalently associate with an apparent stoichiometry of one A and five B chains to form the holotoxin.
The Shiga toxins are a family of related protein toxins secreted by certain types of bacteria. Shiga toxin (Stx) is produced by Shigella dysenteriae; whereas, the Shiga-like toxins, Stx1 and Stx2, with a few known isoforms, are secreted by specific strains of Escherichia coli named Shiga-toxin-producing E. coli (STEC) such as E. coli O157:H7. The latter may cause bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications.

Specificity

Monoclonal Anti-Shiga Toxin 1, B Subunit antibody specifically recognizes the B subunit of Shiga holotoxin.

Immunogen

purified Shiga-like toxin from E.coli H30

Application

Anti-Shiga Toxin 1, B Subunit (STxB) antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • flow cytometry
  • immunoprecipitation

Biochem/physiol Actions

Shiga-toxin (Stx) produced by E. coli O157:H7 may cause bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications. All the Stx isoforms have similar structure and mechanism of action. The catalytic A subunit has RNA N-glycosidase activity that inhibits eukaryotic protein synthesis. The B subunits form a pentamer, which recognizes and binds to the functional cell-surface receptor globotriaosylceramide [Gb3, Gala(1-4)-Galb (1-4)-Glcb1-ceramide]. Gb3 is overexpressed in membranes of numerous tumor cells, Therefore, STxB binding to Gb3 receptors may be useful for cell-specific vectorization, labeling, and imaging purposes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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In vivo tumor targeting by the B-subunit of shiga toxin
Viel T, et al.
Molecular Imaging, 7(6), 7290-2008 (2008)
Shiga toxin and its use in targeted cancer therapy and imaging
Engedal N, et al.
Microbial Biotechnology, 4(1), 32-46 (2011)
Human colorectal tumors and metastases express Gb3 and can be targeted by an intestinal pathogen-based delivery tool
Falguieres T, et al.
Molecular Cancer Therapeutics, 7(8), 2498-2508 (2008)
Shiga toxin: purification, structure, and function
Donohue-Rolfe A, et al.
Reviews of Infectious Diseases, 13, S293-S297 (1991)

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