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R9782

Sigma-Aldrich

RS-1

≥98% (HPLC)

Synonym(s):

3-[(benzylamino)sulfonyl]-4-bromo-N-(4-bromophenyl)benzamide, 4-Bromo-N-(4-bromophenyl)-3-[[(phenylmethyl)amino]sulfonyl]-benzamide, RAD51-stimulatory compound 1

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About This Item

Empirical Formula (Hill Notation):
C20H16Br2N2O3S
CAS Number:
312756-74-4
Molecular Weight:
524.23
MDL number:
MFCD00348720
UNSPSC Code:
12352200
PubChem Substance ID:
329824048
NACRES:
NA.77

Assay

≥98% (HPLC)

form

powder

color

off-white to light tan

solubility

DMSO: ≥10 mg/mL

storage temp.

room temp

SMILES string

Brc1ccc(NC(=O)c2ccc(Br)c(c2)S(=O)(=O)NCc3ccccc3)cc1

InChI

1S/C20H16Br2N2O3S/c21-16-7-9-17(10-8-16)24-20(25)15-6-11-18(22)19(12-15)28(26,27)23-13-14-4-2-1-3-5-14/h1-12,23H,13H2,(H,24,25)

InChI key

SWKAVEUTKGKHSR-UHFFFAOYSA-N

Application

RS-1 has been shown to enhance CRISPR genome editing efficiency. To see other small molecule CRISPR enhancers, visit sigma.com/CRISPR-enhancers.
RS-1 has been used:
  • as a homology-directed repair (HDR) agonist to study its effects on in human hematopoietic stem/progenitor cells (HSPCs)
  • as HDR agonist to analyze its effects on DNA repair modulation in human induced pluripotent stem (iPS) cells
  • as RAD51 agonist to study its effects on double-stranded break repair

Biochem/physiol Actions

RS-1 (RAD51-stimulatory compound 1) is a stimulator of the human homologous recombination (HR) protein RAD51. RS-1 stimulates binding of hRAD51 to single stranded DNA (ssDNA) and enhances recombinogenic activity by stabilizing the active form of hRAD51 filaments without inhibiting hRAD51 ATPase activity. RS-1 has been shown to enhance CRISPR-Cas9 knock-in efficiency in HEK293A cells and has been shown to enhance both TALEN and Cas9-mediated knock-in efficiency in rabbits.
RS-1 is a sulfonamido-benzamide compound.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Liqian Zhu et al.
Veterinary research, 48(1), 45-45 (2017-09-09)
Bovine herpesvirus 1 (BoHV-1) infection enhanced the generation of inflammatory mediator reactive oxidative species (ROS) and stimulated MAPK signaling that are highly possibly related to virus induced inflammation. In this study, for the first time we show that BoHV-1 infection
Xue Wang et al.
BioMed research international, 2019, 7878906-7878906 (2019-11-07)
It has been reported that paclitaxel administration could cause sensorineural hearing loss, and Wnt activation is important for the development and cell protection of mouse cochlea. However, the effect of Wnt signaling in spiral ganglion neurons (SGNs) damage induced by
Siti Nur Syahirah Ahmad et al.
Foods (Basel, Switzerland), 10(2) (2021-01-31)
This study aims to investigate the effect of different vegetable oils and frying cycles on acrylamide formation during the intermittent frying of beef nuggets. Different vegetable oils, palm olein (PO), red palm olein (RPO), sunflower oil (SFO), and soybean oil
Jon Gil-Martínez et al.
International journal of molecular sciences, 22(4) (2021-03-07)
Fumarylacetoacetate hydrolase (FAH) is the fifth enzyme in the tyrosine catabolism pathway. A deficiency in human FAH leads to hereditary tyrosinemia type I (HT1), an autosomal recessive disorder that results in the accumulation of toxic metabolites such as succinylacetone, maleylacetoacetate
Wenwen Liu et al.
Antioxidants & redox signaling, 30(11), 1389-1410 (2018-03-29)
Cisplatin can damage spiral ganglion neurons (SGNs) and cause sensorineural hearing loss. Wnt activation protects against neomycin-induced hair cell damage in the mouse cochlea, but the role of Wnt signaling in protecting SGNs from cisplatin treatment has not yet been
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