P7130

Puromycin aminonucleoside

• Synonym(s): 3′-Amino-3′-deoxy-N⁶,N⁶-dimethyladenosine
• Empirical Formula (Hill Notation): C₁₂H₁₈N₆O₃
• CAS Number: 58-60-6
• Molecular Weight: 294.31
• Beilstein: 93902
• EC Number: 200-388-3
• MDL number: MFCD00063462
• UNSPSC Code: 51281912
• PubChem Substance ID: 24898823
• NACRES: NA.85

Properties

• form: powder
• Quality Level: 300
• solubility: H₂O: soluble 50 mg/mL
• antibiotic activity spectrum: Gram-positive bacteria; neoplastics; parasites
• Mode of action: protein synthesis | interferes
• storage temp.: 2-8°C
• SMILES string: CN(C)c1ncnc2n(cnc12)[C@@H]3O[C@H](CO)[C@H](N)[C@@H]3O
• InChI: 1S/C12H18N6O3/c1-17(2)10-8-11(15-4-14-10)18(5-16-8)12-9(20)7(13)6(3-19)21-12/h4-7,9,12,19-20H,3,13H2,1-2H3/t6-,7+,9+,12-/m1/s1
• InChI key: RYSMHWILUNYBFW-VENHTOENSA-N

Description

General description

Puromycin aminonucleoside is the aminonucleoside portion of the antibiotic puromycin. It is useful in nephrology research, like studies of focal and segmental glomerulosclerosis and in the induction of nephrosis in rats. The excretion of sodium and nitrite (NOx) metabolites in rats with puromycin aminonucleoside-induced nephrotic syndrome are studied. Puromycin aminonucleoside-induced nephrosis in rats has been studied with respect to the production of reactive oxygen species during the acute phase. Puromycin aminonucleoside is used to probe endothelial glycosaminoglycan synthesis in cultured glomerular endothelial cells and their relation to cell permeability.

Application

Puromycin aminonucleoside has been used:

• as a selection marker for the infected cells for the transfection of lentivirus
• as a component of fresh medium for transfection and infection assays
• to subcutaneously induce puromycin aminonucleoside nephrosis to investigate the mRNA and protein levels of nephrin and podocin before the onset of proteinuria

Biochem/physiol Actions

Puromycin aminonucleoside is used to study human glomerular disease by inducing damage of murine glomerular podocytes and is used to study glomerular function and morphology.

Other Notes

Keep container tightly closed in a dry and well-ventilated place.

Safety Information

• Pictograms: GHS08
• Signal Word: Warning
• Hazard Statements: H373
• Hazard Classifications: STOT RE 2
• Target Organs: Kidney
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves