P3369
Protamine sulfate
solid, 90.0-110.0% dry basis,≥92% (chromatography), suitable for transduction
Synonym(s):
Salmine sulfate from salmon sperm, Protamine sulfate
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Product Name
Protamine sulfate, meets USP testing specifications
Agency
meets USP testing specifications
Quality Level
Assay
≥92% (chromatography)
90.0-110.0% dry basis
form
solid
mol wt
Mr 5000-10000
technique(s)
transduction: suitable
shipped in
ambient
storage temp.
2-8°C
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General description
Protamine sulfate is a cationic polypeptide having a molecular weight of 5 kDa, isolated from salmon sperm.
Application
Protamine sulfate has been used in the transduction of
- lentiviruses into human embryonic 293T cells
- shRNA into mesenchymal stem cells/marrow stromal cells (MSC)
- lenti Sca1-OCT4 plasmid into human cord blood hematopoietic progenitor CD34+ cells
Biochem/physiol Actions
Alkaline protamine binds to negatively charged heparin. Use of protamine during cardiovascular surgery sometime triggers anaphylactic reactions.
Protamine facilitates direct transfection of microbial cells, and of human cells using cationic liposomes. Protamine effectively neutralizes the anticoagulation effects of heparin.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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