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MAK477

Sigma-Aldrich

Calcium Assay Kit

sufficient for 500 colorimetric tests

Synonym(s):

Calcium Quantitative Test Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

100

application(s)

pharmaceutical

detection method

colorimetric

relevant disease(s)

musculoskeletal disorders; genitourinary disorders; cancer; endocrinological disorders

shipped in

ambient

storage temp.

2-8°C

General description

Calcium is measured to monitor diseases of the bone or calcium regulation disorders. Increased calcium levels in serum are reported in hyperparathyroidism, metastatic bone lesions and hypervitaminosis. While decreased levels are observed in hypoparathyroidism, nephrosis, rickets, steatorrhea, nephritis and calcium-losing syndromes. Urinary calcium levels aid in understanding how the kidneys handle calcium in certain diseases of the parathyroid gland. Urinary calcium levels are also essential in the evaluation of kidney stones.

Features and Benefits

  • The linear detection range of the kit is 0.08 mg/dL (20 µM) to 20 mg/dL (5 mM).
  • Compatible with high-throughput handling systems.
  • The optimized formulation has greatly enhanced reagent and signal stability.
  • Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipids, protein, magnesium, iron and zinc.

Suitability

The kit is suitable for calcium determination in blood, urine, saliva and other biological samples, as well as for studying the effects of drugs on calcium metabolism.

Principle

This Calcium Assay Kit is designed to measure calcium directly in biological samples without any pre-treatment. Phenolsulphonephthalein dye in the kit forms a very stable blue colored complex specifically with free calcium. The intensity of the color, measured at 612 nm, is directly proportional to the calcium concentration in the sample. The optimized formulation minimizes any interference by substances such as magnesium, lipids, protein, and bilirubin.

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H314,H335

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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