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I8896

Sigma-Aldrich

IGEPAL® CA-630

for molecular biology

Synonym(s):

Octylphenoxy poly(ethyleneoxy)ethanol, branched

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About This Item

Linear Formula:
(C2H4O)nC14H22O
CAS Number:
9002-93-1
UNSPSC Code:
12352104
NACRES:
NA.31

grade

for molecular biology

description

non-ionic

form

liquid

technique(s)

IR spectroscopy: suitable

CMC

0.08 mM (20-25°C)

HLB

13

foreign activity

DNase and RNase, none detected

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI key

JYCQQPHGFMYQCF-UHFFFAOYSA-N

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General description

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40.

Application

IGEPAL® CA-630 has been used
  • as neutral detergent in homogenizing buffer during western blotting of mouse muscle homogenates
  • In X-gal staining for studying the lacZ expression in E8.5-E10.5 embryos
  • In Cell-Lysis (CL) buffer for preparing RT-qPCR-ready cell lysates

Other Notes

Nonionic, non-denaturing detergent

Quality

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Caution

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

Legal Information

IGEPAL is a registered trademark of Solvay

Signal Word

Danger

Hazard Statements

H302,H315,H318,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

483.8 °F - closed cup

Flash Point(C)

251 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Joseph A Roche et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 59(11), 964-975 (2011-11-02)
Mutations in the DYSF gene that severely reduce the levels of the protein dysferlin are implicated in muscle-wasting syndromes known as dysferlinopathies. Although studies of its function in skeletal muscle have focused on its potential role in repairing the plasma
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HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained
Amina Abbadi et al.
The Journal of biological chemistry, 291(3), 1448-1455 (2015-11-26)
Many cells, including murine airway epithelial cells, respond to a variety of inflammatory stimuli by synthesizing leukocyte-adhesive hyaluronan (HA) cables that remain attached to their cell surfaces. This study shows that air-liquid interface cultures of murine airway epithelial cells (AECs)
Per Wasteson et al.
Development (Cambridge, England), 135(10), 1823-1832 (2008-04-18)
Aortic smooth muscle cells (SMCs) have been proposed to derive from lateral plate mesoderm. It has further been suggested that induction of SMC differentiation is confined to the ventral side of the aorta, and that SMCs later migrate to the
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Sample nucleic acid purification can often be rate-limiting for conventional quantitative PCR (qPCR) workflows. We recently developed high-throughput virus microneutralization assays using an endpoint assessment approach based on reverse transcription qPCR (RT-qPCR). The need for cumbersome RNA purification is circumvented
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Protocols

Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit

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Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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