GE29-0486-31

HisTrap^™ Fast Flow Crude

Cytiva 29-0486-31, pack of 1 mL

• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• packaging: pack of 1 mL
• manufacturer/tradename: Cytiva 29-0486-31
• availability: not available in North America
• parameter: 42 psi (H₂O at room temperature.)
• bed size: 7 mm × 25 mm
• bed volume: 1 mL
• column I.D.: 7 mm
• matrix: 6% cross-linked agarose
• particle size: 45-165 μm
• average diameter: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• working range: 3-12(Ni²⁺-stripped medium.)
• capacity: ~40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.); ~40 mg binding capacity(histidine-tagged protein)
• suitability: suitable for bioprocess medium

Description

General description

HisTrap FF crude columns offer the convenience of simple, one-step purification of histidine-tagged proteins directly from homogenized, unclarified cell lysate by immobilized metal ion affinity chromatography (IMAC).

Application

HisTrap FF crude is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. This prepacked column is intended for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). After thorough cell disruption, it is possible to load the unclarified lysate on the column without precentrifugation and filtration of the sample. Extending the duration of the mechanical treatment of the sample to ensure a more complete lysis is recommended. For optimal results, we recommend first addition of lysozyme and DNase I followed by a mechanical lysis, for example by sonication. The homogenized sample can then be loaded directly on the column without a clarification step which may prevent degradation of the target protein and increase the activity.

Features and Benefits

• Optimized for purification of histidine-tagged proteins directly from homogenized, unclarified cell lysates.
• High binding capacity, approx. 40 mg/ml medium and negligible Ni2+ leakage
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
• Reduced sample preparation time, which minimizes risk of protein degradation by proteases.
• No need for centrifugation or filtration of the samples. Direct sample application.
• Reliable purification of histidine-tagged proteins directly from unclarified lysates–simply sonicate and run.
• Reduced sample preparation time, which minimizes risk of protein degradation by proteases
• Simple operation with a syringe, pump, or chromatography system such as ÄKTAdesign or FPLC System
• Permit rapid yet reliable separations with a minimum of sample preparation and equipment

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HisTrap is a trademark of Cytiva

Safety Information

• Pictograms: GHS09
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids