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GE28-9523-60

GST GraviTrap™

Cytiva 28-9523-60

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About This Item

UNSPSC Code:
41106501
NACRES:
NA.56

ligand

glutathione

packaging

pkg of 10 ea

manufacturer/tradename

Cytiva 28-9523-60

bed volume

2 mL

column I.D.

15 mm

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

4-13

working range

4-13

capacity

≥20 mg binding capacity(recombinant glutathione S-transferase (rGST))
 >25 mg binding capacity (horse liver GST/ml medium)

General description

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
GST GraviTrap™ contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose™ 4B, sufficient for purification of up to 20 mg of fusion protein.

Application

GST GraviTrap™ contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose™ 4B, sufficient for purification of up to 20 mg of fusion Protein. GST-tagged Proteins are recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the Proteins. The binding capacity of prepacked GST GraviTrap™ columns, which can be greater than 10 mg Protein/mL medium, varies with the nature of the tagged Protein and the exact binding conditions used. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged Proteins.

Features and Benefits

  • Convenient, prepacked gravity-fLow columns.
  • Allows direct purification of both clarified and unclarified cell lysates.
  • Yields up to 20 mg of pure Proteins per column.
  • Yields up to 20 mg of pure Proteins per column

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

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This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.

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This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Troubleshooting Strategies in GST-tagged Protein Purification

This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

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Protocols

Characteristics of Glutathione Sepharose® Products

Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service