GE28-9374-52
DIGE Buffer Kit
Cytiva 28-9374-52, pack of 1 kit
About This Item
Recommended Products
sterility
non-sterile
form
liquid
packaging
pack of 1 kit
manufacturer/tradename
Cytiva 28-9374-52
storage temp.
2-8°C
General description
Dige gel should be used with the dige buffer kit, which consists of concentrated running buffers and sealing solution for attaching immobilinedrystrip gels (ipg strips) to the top of the polyacrylamide gel. the capacity of the buffer system is similar to the commonly used laemmli (tris-glycine) buffer system, and the separation performance of dige gel is comparable to 12.5% laemmli gels (1). dige gel and dige buffer kit offer the high quality and performance of the laemmli system with the added advantages of convenience and time savings.
Dige gel is intended for use with the dige buffer kit and ettan dalt large vertical system. ettan dalttwelve and ettan daltsix instruments are designed for large-format, second dimension 2-d electrophoresis using either 18 or 24 cm immobiline drystrip gels. with ettan dalttwelve, up to 12 dige gels can be run simultaneously with separations complete in 4 to 6 h. the dige buffer kit contains the amount of reagents necessary for a single run of up to 12 dige gels using the ettan dalttwelve electrophoresis unit, or two runs of up to six gels using the ettan daltsix unit.
Features and Benefits
- Precast 12.5% polyacrylamide buffer for second-dimension 2-D electrophoresis.
- Ready-to-use products with a long shelf-life that saves you time by eliminating the need to make buffers, cast gels, and wash glass plates, leaving more time for more important work.
- DIGE gels run with DIGE Buffer Kit result in spot maps with higher reproducibility compared to homecast polyacrylamide gels.
Storage and Stability
Analysis Note
Storage Class Code
12 - Non Combustible Liquids
Certificates of Analysis (COA)
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Protocols
This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.
This page describes two-dimensional separation of protein samples with Cytiva products.
This page describes two-dimensional separation of protein samples with Cytiva products.
This page describes two-dimensional separation of protein samples with Cytiva products.
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