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GE17-0978-02

Butyl-s Sepharose™ 6 Fast Flow

Cytiva 17-0978-02, pack of 200 mL

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

butyl-S

packaging

pack of 200 mL

manufacturer/tradename

Cytiva 17-0978-02

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-14

working range

3-13

suitability

suitable for bioprocess medium

General description

Butyl-S Sepharose™ 6 Fast FLow is the least hydrophobic medium in the Sepharose™ Fast FLow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of a purification process to remove the bulk of impurities.

Application

Butyl-S Sepharose™ 6 Fast FLow is part of the Sepharose™ Fast FLow HIC platform, which has been an industrial standard for HIC chromatography during recent decades. Butyl-S Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with butyl groups via a thio-ether linkage. It will exhibit a different selectivity compared to Butyl Sepharose™ 4 Fast FLow and other HIC Sepharose™ Fast FLow products.

This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively Low salt concentrations. Butyl?S Sepharose™ 6 Fast FLow is particularly useful during the initial stages of a separation process to remove the bulk of impurities without stringent requirements for conditioning of the sample (e.g. adjustment of pH, salt concentration etc.)

Butyl-S Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Butyl-S Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Binds and elutes relatively strong hydrophobic molecules at comparatively Low salt concentrations
  • The least hydrophobic medium in the well proven Sepharose™ Fast FLow HIC range
  • Purifies recombinant Hepatitis B virus surface antigen from CHO cells
  • Developed and optimized in cooperation with a process-scale manufacturer of biopharmaceuticals
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Salt Selection & Buffer Preparation

Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.

Salt Selection and Buffer Preparation

Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.

Salt Selection & Buffer Preparation

Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.

Salt Selection & Buffer Preparation

Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.

See All

Protocols

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service