GE17-0300-10

Ficoll^® PM 400

Cytiva 17-0300-10, pack of 100 g

• Synonym(s): Density gradient medium
• UNSPSC Code: 12352201
• NACRES: NA.25

Properties

• form: solid; spray-dried
• packaging: pack of 100 g
• manufacturer/tradename: Cytiva 17-0300-10
• color: White
• solubility: soluble

Description

General description

High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. The molecules are highly branched and the high content of hydroxyl groups leads to very good solubility in aqueous media.

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained

Features and Benefits

• For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
• Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.
• Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
• Better osmotic properties than sucrose.
• Preserves functional and morphological integrity.
• Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
• Does not penetrate biological membranes.
• Serves as the raw material for preparation of Ficoll-Paque gradients.
• Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Ficoll is a registered trademark of Cytiva

Safety Information

• Storage Class Code: 13 - Non Combustible Solids