G1924
L-Glutamate Oxidase from Streptomyces sp.
recombinant, expressed in E. coli
Synonym(s):
L-Glutamate:oxygen oxidoreductase (deaminating)
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About This Item
Recommended Products
recombinant
expressed in E. coli
Quality Level
form
solid
specific activity
≥5 units/mg protein
mol wt
120 kDa
storage temp.
−20°C
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General description
L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa. The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group.
This product has a 6xHistidine tag with a thrombin cleavage site.
Application
L-Glutamate Oxidase from Streptomyces sp may be used:
- as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor development
- as a component of pencil graphite electrode for biosensing L-glutamate
Biochem/physiol Actions
L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to α-ketoglutarate, ammonia and hydrogen peroxide. LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits.
Unit Definition
One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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