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Key Documents

E1894

Sigma-Aldrich

EPPS

BioXtra, ≥99.5% (titration)

Synonym(s):

Triethanolamine hydrochloric acid salt, 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid, 4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), HEPPS

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About This Item

Empirical Formula (Hill Notation):
C9H20N2O4S
CAS Number:
Molecular Weight:
252.33
Beilstein:
3957385
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

product line

BioXtra

Quality Level

Assay

≥99.5% (titration)

form

powder

impurities

≤0.005% Phosphorus (P)
≤0.1% Insoluble matter

ign. residue

≤0.1%

pH

5.0-7.0 (20 °C, 1 M in H2O)

useful pH range

7.3-8.7

pKa 

8

mp

237-239 °C (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

bromide (Br-): ≤0.1%

cation traces

Al: ≤0.0005%
Ca: ≤0.002%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.1%
Na: ≤0.005%
Pb: ≤0.001%
Zn: ≤0.0005%

absorption

≤0.1 at 260 in H2O at 1 M
≤0.1 at 280 in H2O at 1 M

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCCS(O)(=O)=O)CC1

InChI

1S/C9H20N2O4S/c12-8-7-11-5-3-10(4-6-11)2-1-9-16(13,14)15/h12H,1-9H2,(H,13,14,15)

InChI key

OWXMKDGYPWMGEB-UHFFFAOYSA-N

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General description

EPPS, also known as 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid, is a zwitterionic buffering agent that belongs to the group of good buffers. It plays a vital role in maintaining a stable pH in solutions, which is critical for various biological and biochemical processes. EPPS buffer finds extensive application in biological research and cell culture experiments.

Application

EPPS buffer has been used as a component of buffer solution for methylglyoxal (MG) treatment in vitro to form advanced glycation end products (AGEs) to crosslink collagen fibrils. It has also been used in the preparation of fixation buffer solution for light microscopy.

Features and Benefits

  • The BioXtra product line ensures T15 high-quality products for consistent results.
  • A high assay of ≥99.5% guarantees the purity of the product.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Effects of maturation and advanced glycation on tensile mechanics of collagen fibrils from rat tail and Achilles tendons
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Shrivastava A, et al.
Applied Mechanics and Materials, 176, 104524-104524 (2023)
lonization Enthalpies of Some Common Zvvitterionic Hydrogen-lon Buffers (HEPES, PIPES, HEPPS and BES) for Biological Research
Roig T, et al.
Journal of Separation Science, 993(47), 899-90j-899-90j (1993)
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