Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

D3404

Sigma-Aldrich

Plasmid DNA from E. coli RRI

pUC19, buffered aqueous solution

Sign Into View Organizational & Contract Pricing


About This Item

CAS Number:
MDL number:
UNSPSC Code:
41105600
NACRES:
NA.85

grade

for molecular biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

The pUC19 plasmid (2,686 bp) confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the B-galactosidase gene; the plasmid has thirteen unique sites in the MCS (Acc I, BamH I, EcoR I, Hinc II, Hind III, Kpn I, Pst I, Sac I, Sal I, Sma I, Sph I, Xba I, and Xma I).

Application

Plasmid DNA from Escherichia coli RRI has been used for imaging of DNA (nanostructure) via atomic force microscopy.

Biochem/physiol Actions

These plasmids confer ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the β-galactosidase gene; pUC8 and 9 have nine unique sites within the MCS while pUC18 and 19 have thirteen.
Foreign DNA inserted at the MCS abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium. The orientations of the MCS regions in the pUC plasmids are analogous to those of the corresponding M13 phage.

Components

pUC19 Plasmid DNA, 10 μg is supplied at approximately 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Principle

Foreign DNA inserted at the MCS interrupts the β-galactosidase gene and abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Measurement of DNA Morphological Parameters at Highly Entangled Regime on Surfaces
Annalisa Calo
The Journal of Physical Chemistry (2009)
Atomic force microscopy with inherent
disturbance suppression for nanostructure
imaging
A W Sparks
Nanotechnology (2006)
J Zeyland et al.
Polish journal of veterinary sciences, 16(2), 265-273 (2013-08-27)
Extinct aurochs (Bos primigenius), accepted as the ancestor of domestic cattle, was one of the largest wild animals inhabiting Europe, Asia and North Africa. The gradual process of aurochs extinction finished in Poland in 1627, were the last recorded aurochs
Nicola Carboni et al.
Acta myologica : myopathies and cardiomyopathies : official journal of the Mediterranean Society of Myology, 32(1), 7-17 (2013-07-16)
Mutations on the LMNA gene are responsible for an heterogeneous group of diseases. Overlapping syndromes related to LMNA gene alterations have been extensively reported. Study scope is to perform a systematic analysis of the overlapping syndromes so far described and
[Transcription factor Kaiso does not interact with hydroxymethylated DNA within CTGCNA sequence context].
S V Zhenilo et al.
Molekuliarnaia biologiia, 47(3), 522-525 (2013-07-31)

Protocols

Preparation for biodegradable nanoparticles and their use in transfection protocols .

Preparation for biodegradable nanoparticles and their use in transfection protocols .

Preparation for biodegradable nanoparticles and their use in transfection protocols .

Preparation for biodegradable nanoparticles and their use in transfection protocols .

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service