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CS0013

Sigma-Aldrich

Red Protease Detection Kit

Fluorescent protease assay, high sensitivity

Synonym(s):

Fluorescent Protease Assay Kit, Protease Assay Kit

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About This Item

UNSPSC Code:
12350000
NACRES:
NA.51

Quality Level

200

packaging

pkg of 1 kit

shipped in

dry ice

storage temp.

−20°C

General description

The Red Protease Detection Kit uses TAMRA-casein as a convenient fluorescent substrate for detection of proteases in various samples.

Fluorescence excitation wavelength = 535 nm

Fluorescence emission wavelength = 595 nm

Application

Detection and quantitation of protease activity in samples. Useful for impurity testing.

Features and Benefits

  • Fluorescence is pH-independent
  • No separation or precipation steps are required
  • High sensitivity and signal-to-noise

Components

CS0013A Trypsin (1 mg/mL): 100 μL

CS0013B Protease AssayBuffer × 2: 30 mL

CS0013C TAMRA-Casein substrate: 280 μL

Suitability

Detection limit approximately 19 ng/ml, when using a 1 hour incubation with Trypsin as a standard.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Better Protease Detection | High-Sensitivity Protease Detection Assay

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

Better Protease Detection | High-Sensitivity Protease Detection Assay

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

Better Protease Detection | High-Sensitivity Protease Detection Assay

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

Better Protease Detection | High-Sensitivity Protease Detection Assay

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

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